| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protocadherin-20, Protocadherin-13, PCDH20, PCDH13 |
| Entrez GeneID | 64881 |
| WB Predicted band size | 104.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PCDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-472 amino acids from the Central region of human PCDH20. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PCDH20抗体的3篇示例文献(内容为模拟概括，实际引用需核对原文)：
1. **文献名称**：*Protocadherin-20 suppresses tumorigenesis via inhibition of Wnt/β-catenin signaling in colorectal cancer*
**作者**：Li Y, et al.
**摘要**：研究利用PCDH20抗体检测其在结直肠癌组织中的表达水平，发现PCDH20通过抑制Wnt/β-catenin通路抑制肿瘤生长，低表达与患者预后不良相关。
2. **文献名称**：*Epigenetic silencing of PCDH20 promotes hepatocellular carcinoma cell invasion*
**作者**：Wang X, et al.
**摘要**：通过免疫组化(使用PCDH20抗体)发现肝癌中PCDH20因启动子甲基化而下调，其缺失通过激活ERK通路增强癌细胞侵袭能力。
3. **文献名称**：*PCDH20 as a candidate tumor suppressor in gastric cancer: Correlation with clinicopathological features*
**作者**：Zhang H, et al.
**摘要**：利用Western blot和免疫荧光(基于PCDH20抗体)证实PCDH20在胃癌中表达显著降低，且与淋巴结转移和晚期分期呈负相关，提示其抑癌作用。
**备注**：以上为示例文献框架，实际文献需通过PubMed或学术数据库检索确认，部分研究可能聚焦于基因调控机制而非抗体直接应用。
The protocadherin 20 (PCDH20) antibody is a tool used to detect PCDH20. a member of the protocadherin family involved in cell-cell adhesion and signaling. Protocadherins are calcium-dependent transmembrane proteins critical for neural development, tissue morphogenesis, and cellular recognition. PCDH20. located on chromosome 13. is classified as a tumor suppressor due to its frequent epigenetic silencing in cancers. Studies link its downregulation to promoter hypermethylation in malignancies like colorectal, breast, and hepatocellular carcinomas, where loss of PCDH20 correlates with enhanced metastatic potential, poor prognosis, and dysregulated Wnt/β-catenin signaling.
The PCDH20 antibody is primarily utilized in research to study protein expression patterns in normal and diseased tissues. It aids in identifying PCDH20's role in maintaining epithelial integrity, suppressing invasion, and modulating cell proliferation. Applications include immunohistochemistry (IHC), Western blot (WB), and immunofluorescence (IF) to localize PCDH20 in cellular or tissue samples. Commercially available antibodies are typically raised in rabbits or mice, targeting specific epitopes of human PCDH20. Validation involves testing reactivity across species and confirming reduced expression in methylated cancer cell lines.
Research using PCDH20 antibodies contributes to understanding its mechanistic role in carcinogenesis and potential as a biomarker for early detection or therapeutic targeting. However, variability in antibody specificity and experimental conditions necessitates careful optimization in different assays.
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