WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Endonuclease 8-like 3, 322-, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3, NEIL3 |
Entrez GeneID | 55247 |
WB Predicted band size | 67.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This NEIL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-320 amino acids from the Central region of human NEIL3. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NEIL3抗体的模拟参考文献示例(仅供学术参考,建议通过PubMed或SciHub等平台验证文献真实性):
1. **"NEIL3 DNA glycosylase regulates hematopoietic stem cell homeostasis"**
*作者:Zhang Y, et al. (2021)*
摘要:研究通过NEIL3抗体检测其在造血干细胞中的表达及定位,发现NEIL3通过修复氧化损伤DNA维持干细胞基因组稳定性,缺失导致造血功能障碍。
2. **"A role for NEIL3 in promoting glioblastoma resistance to chemotherapy"**
*作者:Wang L, et al. (2020)*
摘要:利用NEIL3抗体进行蛋白印迹分析,证明胶质母细胞瘤中NEIL3高表达与其化疗耐药相关,敲低NEIL3可增强肿瘤细胞对替莫唑胺的敏感性。
3. **"NEIL3-dependent telomere repair during mitosis safeguards genome integrity"**
*作者:Sørensen CS, et al. (2019)*
摘要:通过免疫荧光结合NEIL3抗体,发现NEIL3在细胞有丝分裂期修复端粒氧化损伤,防止染色体断裂,揭示其不同于其他糖基化酶的独特功能。
4. **"Development and validation of a monoclonal NEIL3 antibody for immunohistochemical analysis"**
*作者:Tanaka K, et al. (2022)*
摘要:报道一种高特异性NEIL3单克隆抗体的开发,验证其在人组织切片中的免疫组化适用性,并发现NEIL3在结肠癌组织中的异常高表达现象。
注:以上为模拟文献,实际研究请查阅具体数据库。NEIL3相关研究多集中于DNA修复机制、癌症及干细胞领域,建议结合关键词“NEIL3 antibody application”或“NEIL3 immunohistochemistry”进一步检索。
The NEIL3 (Center) antibody is designed to target the central region of the NEIL3 protein, a key enzyme in DNA repair processes. NEIL3 (Endonuclease VIII-like 3) belongs to the NEIL family of DNA glycosylases, which play critical roles in the base excision repair (BER) pathway by recognizing and excising oxidized or damaged bases, such as oxidative lesions (e.g., hydantoins, spiroiminodihydantoin) and intra-strand crosslinks. Unlike other NEIL family members (NEIL1 and NEIL2), NEIL3 exhibits unique substrate specificity and is particularly active during the S phase of the cell cycle, suggesting a specialized role in replication-associated DNA repair. It is also implicated in resolving complex DNA lesions at replication forks and in telomere maintenance.
NEIL3 is highly expressed in proliferating cells, including stem cells and certain cancer types, linking it to cellular proliferation, genome stability, and oncogenesis. Studies highlight its potential role in neurodevelopment, immune regulation, and response to chemotherapy. The NEIL3 (Center) antibody, often validated in applications like Western blotting, immunohistochemistry, or immunofluorescence, enables researchers to study NEIL3's expression, localization, and function. Its specificity for the central domain ensures detection of full-length or truncated variants, aiding investigations into NEIL3's regulatory mechanisms and its association with diseases like cancer, neurodegeneration, or aging-related disorders.
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