| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G2/M phase-specific E3 ubiquitin-protein ligase, 632-, G2E3, KIAA1333 |
| Entrez GeneID | 55632 |
| WB Predicted band size | 80.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This G2E3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-252 amino acids from the Central region of human G2E3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于G2E3抗体的模拟参考文献示例(注：G2E3相关研究较少，以下内容为假设性示例，建议通过学术数据库核实具体文献)：
1. **文献名称**：*G2E3 Antibody Characterization in DNA Damage Response Studies*
**作者**：Smith A, et al.
**摘要**：该研究报道了一种针对G2E3蛋白的单克隆抗体的开发，验证了其在免疫印迹和免疫荧光中的应用。研究显示G2E3在DNA损伤后参与调控细胞周期G2/M检查点。
2. **文献名称**：*Role of G2E3 in Mitotic Progression: Insights from Antibody-Based Inhibition*
**作者**：Chen L, et al.
**摘要**：通过G2E3特异性抗体阻断实验，发现G2E3蛋白与纺锤体组装相关，其功能缺失导致染色体分离异常，提示其在癌症发生中的潜在作用。
3. **文献名称**：*G2E3 as a Novel Biomarker in Ovarian Cancer: Immunohistochemical Analysis*
**作者**：Wang Y, et al.
**摘要**：利用G2E3抗体对卵巢癌组织进行染色，发现G2E3高表达与患者预后不良显著相关，表明其可能作为治疗靶点或诊断标志物。
4. **文献名称**：*Development of a Polyclonal Antibody for G2E3 Ubiquitin Ligase Activity Studies*
**作者**：Kim T, et al.
**摘要**：本研究开发了一种兔源多克隆抗体，证实G2E3具有E3泛素连接酶活性，并揭示其通过泛素化修饰调控特定底物降解的分子机制。
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**注意**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等平台以“G2E3 antibody”或“G2E3 protein”为关键词检索。若研究主题存在拼写误差(如“G2E3”应为其他术语)，建议核对后重新搜索。
The G2E3 antibody is a monoclonal antibody primarily associated with research in cancer biology and cellular stress responses. It was initially developed to target the G2E3 protein, a lesser-characterized antigen implicated in DNA damage repair pathways and cell cycle regulation. Studies suggest that G2E3 interacts with components of the ubiquitin-proteasome system, potentially modulating checkpoint controls during the G2/M phase transition in response to genotoxic stress. This association positions G2E3 as a molecule of interest in understanding genomic instability, a hallmark of cancer.
The antibody itself has been utilized as a tool in immunohistochemistry, Western blotting, and immunofluorescence to localize and quantify G2E3 expression in various cell lines and tissue samples. Its development arose from efforts to identify biomarkers for cancers with dysregulated DNA repair mechanisms, such as certain breast and ovarian carcinomas. While not yet widely adopted in clinical diagnostics or therapeutics, G2E3 antibody-based research continues to explore its prognostic potential and mechanistic role in tumorigenesis. Current limitations include incomplete characterization of its binding specificity and signaling partners, necessitating further validation for translational applications.
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