Rabbit Polyclonal MouseNlrp6 Antibody

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     All lanes : Anti-Mouse Nlrp6 Antibody (Center) at 1:500 dilution Lane 1: Mouse small intestine lysate Lane 2: Mouse heart lysate Lane 3: Mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     All lanes : Anti-Mouse Nlrp6 Antibody (Center) at 1:1000 dilution Lane 1: Mouse small intestine lysate Lane 2: Mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     All lanes : Anti-Mouse Nlrp6 Antibody (Center) at 1:500 dilution Lane 1: Mouse small intestine lysate Lane 2: Mouse liver lysate Lane 3: Mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using P34726 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature,  antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

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     Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using P34726 performed on the Leica® BOND RXm.  Tissue was fixed with formaldehyde at room temperature,   antigen retrieval was by heat mediation with a EDTA buffer (pH9.  0).  Samples were incubated with primary antibody(1:500) for 1 hours at room temperature.   A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

Mouse NLRP6 (NACHT, LRR, and PYD domains-containing protein 6) is a member of the NOD-like receptor (NLR) family, which plays a critical role in innate immunity by functioning as a pattern recognition receptor (PRR). It is primarily expressed in intestinal epithelial cells and immune cells, where it contributes to the formation of inflammasomes—multiprotein complexes that activate caspase-1 and drive the maturation of pro-inflammatory cytokines like IL-18. NLRP6 has been implicated in maintaining mucosal homeostasis, regulating gut microbiota composition, and modulating host defense against pathogens. Studies in mice have shown that NLRP6 deficiency can lead to dysbiosis, increased susceptibility to colitis, and altered responses to metabolic or inflammatory challenges.

Mouse NLRP6 antibodies are essential tools for investigating these biological processes. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect NLRP6 expression and localization in tissues or cells. These antibodies help researchers explore NLRP6's regulatory mechanisms in diseases like inflammatory bowel disease (IBD), metabolic syndrome, and neuroinflammation. However, functional studies have revealed context-dependent roles of NLRP6. with some reports suggesting it may also suppress NF-κB and MAPK signaling pathways. Due to its complex interplay with microbiota and immune signaling, high-specificity anti-NLRP6 antibodies are critical for distinguishing its activity from other NLRs and validating findings in knockout mouse models.