Rabbit Polyclonal MouseNkx2-5 Antibody

The mouse Nkx2-5 antibody is a critical tool for studying cardiac development and related pathologies. Nkx2-5. also known as NK2 homeobox 5 or CSX, is a transcription factor belonging to the NKX family of homeodomain-containing proteins. It plays a pivotal role in early heart development, including cardiomyocyte differentiation, heart tube formation, and chamber specification. In mice, Nkx2-5 is expressed in cardiac progenitor cells and persists in adult cardiomyocytes, regulating genes involved in heart function. Knockout studies in mice demonstrate embryonic lethality due to severe heart malformations, underscoring its essential role in cardiogenesis. The antibody specifically detects mouse Nkx2-5 protein, enabling researchers to visualize its expression and localization in tissues or cells via techniques like immunohistochemistry, Western blotting, or immunofluorescence. It is widely used in cardiac lineage tracing, congenital heart disease models, and studies of transcriptional networks in heart development. Validation typically includes testing in Nkx2-5 knockout controls to confirm specificity. Cross-reactivity with homologs in other species (e.g., human, rat) varies depending on antibody design. Researchers utilize this antibody to explore molecular mechanisms in cardiac regeneration, stem cell differentiation, and disease pathways linked to Nkx2-5 mutations, such as septal defects and conduction abnormalities.