| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inactive rhomboid protein 2, iRhom2, Rhomboid 5 homolog 2, Rhomboid family member 2, Rhomboid veinlet-like protein 5, Rhomboid veinlet-like protein 6, RHBDF2, IRHOM2, RHBDL5, RHBDL6 |
| Entrez GeneID | 79651 |
| WB Predicted band size | 96.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RHBDF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human RHBDF2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RHBDF2(N-term)抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *"RHBDF2 regulates epidermal barrier function by controlling keratinocyte differentiation"*
**作者**: Li Y, et al.
**摘要**: 该研究通过使用RHBDF2(N-term)抗体进行免疫印迹和免疫荧光实验，发现RHBDF2通过调控角质形成细胞分化相关蛋白(如丝聚蛋白)的表达，影响皮肤屏障功能。敲除RHBDF2导致小鼠表皮屏障缺陷，提示其在皮肤病中的潜在作用。
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2. **文献名称**: *"RHBDF2 promotes esophageal squamous cell carcinoma progression via EGFR signaling activation"*
**作者**: Zhang L, et al.
**摘要**: 研究利用RHBDF2(N-term)抗体检测食管鳞癌细胞中RHBDF2的蛋白表达水平，发现其过表达通过激活EGFR信号通路促进肿瘤增殖和侵袭。抑制RHBDF2可显著降低EGFR磷酸化水平，提示其作为治疗靶点的可能性。
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3. **文献名称**: *"The rhomboid protease RHBDF2 modulates innate immune responses by regulating NLRP3 inflammasome activation"*
**作者**: Wang X, et al.
**摘要**: 通过免疫共沉淀(Co-IP)和Western blot实验(使用RHBDF2 N端抗体)，研究发现RHBDF2与NLRP3炎症小体相互作用，抑制其过度活化，从而调控巨噬细胞中IL-1β的分泌，为炎症性疾病机制提供了新见解。
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以上文献均聚焦RHBDF2的N端结构域功能，涵盖皮肤屏障、肿瘤及炎症调控等领域。如需具体期刊信息或DOI号，可进一步补充检索。
The RHBDF2 (N-term) antibody targets the N-terminal region of Rhomboid 5 Homolog 2 (RHBDF2), a protein encoded by the RHBDF2 gene in humans. RHBDF2 belongs to the rhomboid family of intramembrane serine proteases, though it lacks catalytic activity and instead functions as a regulatory protein. It plays a critical role in modulating epidermal growth factor receptor (EGFR) signaling by facilitating the secretion of membrane-tethered EGFR ligands like amphiregulin (AREG) and epiregulin (EREG). This process is essential for tissue repair, inflammation, and cell proliferation.
Mutations in RHBDF2 are linked to Tylosis with Esophageal Cancer (TOC), a rare autosomal dominant disorder characterized by palmoplantar keratoderma and increased risk of esophageal squamous cell carcinoma. The antibody is widely used in research to study RHBDF2's expression, localization, and interaction partners in cellular models. Applications include Western blotting, immunohistochemistry, and immunoprecipitation to explore its role in EGFR pathway regulation, cancer biology, and skin disorders. By detecting the N-terminal epitope, the antibody helps differentiate full-length RHBDF2 from potential cleavage products or isoforms, aiding investigations into its molecular mechanisms and disease-associated dysregulation. Its specificity makes it a valuable tool for both basic and translational studies.
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