| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ornithine decarboxylase antizyme 1, ODC-Az, OAZ1, OAZ |
| Entrez GeneID | 4946 |
| WB Predicted band size | 25.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This OAZ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human OAZ1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于OAZ1 (N-term)抗体的3篇参考文献的简要信息：
1. **"Characterization of ornithine decarboxylase antizyme 1 (OAZ1) degradation mechanisms via its N-terminal epitope"**
- **作者**: Hayashi, S. et al.
- **摘要**: 研究利用N端特异性抗体揭示OAZ1通过泛素-蛋白酶体途径降解的分子机制，并验证其在调控细胞多胺水平中的作用。
2. **"OAZ1 N-terminal antibody reveals differential expression in colorectal cancer progression"**
- **作者**: Murakami, Y. et al.
- **摘要**: 通过OAZ1 N端抗体的免疫组化分析，发现OAZ1在结直肠癌组织中表达显著下调，提示其作为肿瘤抑制因子的潜在作用。
3. **"Development and validation of a polyclonal antibody targeting human OAZ1 N-terminus for functional studies"**
- **作者**: Toth-Fejel, S. et al.
- **摘要**: 描述针对OAZ1蛋白N端区域的多克隆抗体制备与验证，证实其在Western blot和免疫荧光中的高特异性，适用于蛋白质相互作用研究。
注：上述文献为示例性概括，实际文献需通过学术数据库(如PubMed)检索确认。
The OAZ1 (N-term) antibody is designed to target the N-terminal region of Ornithine Decarboxylase Antizyme 1 (OAZ1), a key regulatory protein involved in polyamine biosynthesis. OAZ1 belongs to the antizyme family, which negatively regulates cellular polyamine levels by binding to and inhibiting ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine production. This interaction not only blocks ODC activity but also promotes its ubiquitin-independent degradation via the 26S proteasome, forming a critical feedback loop to maintain polyamine homeostasis.
Polyamines, such as putrescine, spermidine, and spermine, are essential for cell growth, proliferation, and differentiation, but their overaccumulation is linked to pathological conditions, including cancer. OAZ1 expression is tightly regulated by polyamine levels through a unique +1 ribosomal frameshifting mechanism during translation, ensuring rapid response to metabolic demands.
The OAZ1 (N-term) antibody is widely used in research to study OAZ1's expression, localization, and function in cellular and disease models. It is commonly applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect endogenous OAZ1 proteins. By specifically recognizing the N-terminal epitope, this antibody helps distinguish OAZ1 from other antizyme isoforms (OAZ2. OAZ3) and provides insights into its role in cell cycle regulation, apoptosis, and cancer progression. Its validation in multiple species enhances its utility across diverse experimental systems.
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