| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF |
| Entrez GeneID | 9037 |
| WB Predicted band size | 120.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of mouse Sema5a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Sema5a (N-term)抗体的参考文献示例(虚构内容，仅供格式参考)：
1. **标题**: "Semaphorin 5A N-terminal Antibody Identifies Axonal Guidance Cues in Developing Cortex"
**作者**: Tanaka K, et al.
**摘要**: 研究利用抗Sema5a (N-term)抗体，通过免疫组化分析小鼠胚胎皮层，揭示Sema5a在轴突导向中的空间表达模式及其与神经回路形成的关系。
2. **标题**: "Sema5A Cleavage and Tumor Metastasis: Role of N-terminal Epitope Recognition"
**作者**: Müller R, et al.
**摘要**: 使用抗Sema5a (N-term)抗体检测癌细胞中Sema5a的蛋白水解片段，发现其与肿瘤侵袭性相关，提示该抗体在癌症生物标志物研究中的价值。
3. **标题**: "A Novel Monoclonal Antibody Targeting Sema5A N-domain Modulates Synaptic Plasticity"
**作者**: Park S, et al.
**摘要**: 开发并验证了一种高特异性抗Sema5a N端抗体，证实其可通过阻断Sema5a与PSD-95的相互作用影响突触可塑性。
4. **标题**: "Sema5A in Autism Spectrum Disorder: Western Blot Analysis Using N-terminal Antibody"
**作者**: Gonzalez LM, et al.
**摘要**: 通过抗Sema5a (N-term)抗体的Western blot，发现自闭症模型小鼠脑组织中Sema5a表达异常，提示其参与神经发育障碍病理机制。
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*注：以上文献为模拟内容，实际引用需查询真实数据库(如PubMed)。若需具体文献，建议提供抗体生产商货号或详细靶标信息进一步检索。*
The **M Sema5a (N-term) antibody** is a monoclonal antibody specifically designed to target the N-terminal region of Semaphorin-5A (Sema5A), a member of the semaphorin family. Semaphorins are membrane-bound or secreted proteins involved in axonal guidance, cell migration, angiogenesis, and immune regulation. Sema5A uniquely contains seven thrombospondin type-1 repeats (TSRs) in its extracellular domain, enabling interactions with cell surface receptors like plexins and integrins. It plays dual roles in cancer, acting as either a tumor suppressor or promoter depending on context, and is implicated in neurodevelopmental disorders such as autism spectrum disorder (ASD).
The N-terminal region of Sema5A is critical for its functional interactions, including receptor binding and signal modulation. The M Sema5a (N-term) antibody is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study Sema5A expression, localization, and function in tissues or cultured cells. Its specificity for the N-terminal epitope makes it valuable for distinguishing Sema5A from other semaphorin family members. Research applications include investigating Sema5A's role in neuronal circuit formation, tumor microenvironment regulation, and metastasis. Validation in multiple species (e.g., human, mouse) enhances its utility across experimental models. This antibody has contributed to studies linking Sema5A dysregulation to neurological diseases and cancer progression, underscoring its importance as a tool for both basic and translational research.
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