Rabbit Polyclonal TUBB2B(N-term) Antibody

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     All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature,  antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

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     Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature,  antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

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     Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm.  Tissue was fixed with formaldehyde at room temperature,   antigen retrieval was by heat mediation with a EDTA buffer (pH9.  0).  Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature.   A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

The TUBB2B (N-term) antibody is a specialized tool used to detect the N-terminal region of the β-tubulin isoform TUBB2B, a member of the β-tubulin family critical for microtubule formation. Microtubules, dynamic cytoskeletal components, are essential for cell structure, division, intracellular transport, and neuronal development. TUBB2B is predominantly expressed in the central nervous system, particularly in differentiating neurons, where it contributes to microtubule dynamics during processes like axon guidance and cortical migration.

This antibody is widely employed in neuroscience and cell biology research to study TUBB2B expression, localization, and function. Applications include Western blotting, immunohistochemistry, and immunofluorescence to investigate neurodevelopmental disorders, neuronal migration defects, and microtubule-related pathologies. Mutations in the *TUBB2B* gene are linked to malformations of cortical development, such as polymicrogyria and asymmetric dysmorphic cortices, making the antibody valuable for mechanistic studies and diagnostics.

The N-terminal specificity of the antibody helps distinguish TUBB2B from other β-tubulin isoforms (e.g., TUBB2A or TUBB3), reducing cross-reactivity. Researchers also use it to explore microtubule stability, cytoskeletal organization, and cellular responses to microtubule-targeting drugs. By enabling precise detection, the TUBB2B (N-term) antibody serves as a critical tool for unraveling the role of microtubule dynamics in health and disease.