Rabbit Polyclonal ENASE Antibody

ENASE (Endonuclease antibody) typically targets enzymes involved in DNA or RNA cleavage, such as restriction endonucleases or apoptotic nucleases (e.g., EndoG). These enzymes play critical roles in DNA repair, replication, recombination, and programmed cell death. In research, ENASE antibodies are used to study enzymatic mechanisms, monitor protein expression, and investigate diseases linked to DNA damage or dysregulated apoptosis, such as cancer, neurodegeneration, or autoimmune disorders. For example, antibodies against EndoG help explore its role in mitochondrial DNA degradation during apoptosis. ENASE antibodies are often validated for applications like Western blotting, immunofluorescence, or immunohistochemistry, aiding in cellular localization and quantification. Their development relies on immunizing hosts with purified endonuclease fragments or synthetic peptides, followed by affinity purification. Challenges include ensuring specificity due to structural similarities among nucleases. Recent studies leverage these antibodies to dissect CRISPR-associated nucleases or therapeutic targets, emphasizing their utility in molecular biology and translational research.