| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH |
| Entrez GeneID | 642 |
| WB Predicted band size | 52.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This BLMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the Central region of human BLMH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与BLMH(Bleomycin Hydrolase)抗体相关的文献示例及其简要摘要(注：文献信息为模拟示例，非真实存在)：
1. **文献名称**：*Characterization of BLMH Enzyme Activity and Antibody Development in Lung Cancer*
**作者**：Smith A, et al. (2015)
**摘要**：研究开发了高特异性BLMH多克隆抗体，验证其在肺癌组织中的表达，发现BLMH活性升高与博莱霉素耐药性相关，为化疗敏感性预测提供潜在标志物。
2. **文献名称**：*Structural Insights into BLMH via Cryo-EM and Its Immunodetection in Neurodegenerative Models*
**作者**：Johnson R, et al. (2020)
**摘要**：通过冷冻电镜解析BLMH蛋白结构，并利用单克隆抗体证实其在阿尔茨海默病模型小鼠脑组织中的异常聚集，提示其可能参与β-淀粉样蛋白代谢调控。
3. **文献名称**：*BLMH Knockout Mice Reveal Its Role in Drug Metabolism Using Antibody-Based Tissue Staining*
**作者**：Chen L, et al. (2018)
**摘要**：构建BLMH基因敲除小鼠模型，通过免疫组化(使用兔源BLMH抗体)发现BLMH缺失导致博莱霉素毒性增强，证实其在药物解毒中的关键作用。
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**注**：以上为模拟文献，实际研究中建议通过PubMed或Google Scholar以关键词“BLMH antibody”、“Bleomycin Hydrolase detection”检索真实文献。
**Background of BLMH Antibody**
Bleomycin hydrolase (BLMH), also known as bleomycin hydrolase or BMH, is a cytosolic cysteine protease involved in the detoxification of bleomycin, a glycopeptide antibiotic used in cancer chemotherapy. BLMH hydrolyzes the β-aminoalanine-amide bond in bleomycin, neutralizing its DNA-cleaving activity and reducing drug-induced toxicity. Beyond its role in drug metabolism, BLMH participates in cellular processes such as protein turnover, inflammation, and oxidative stress response. It is widely expressed in human tissues, including the liver, lung, and brain.
BLMH antibodies are immunological tools designed to detect and quantify BLMH protein levels in research applications. These antibodies are critical for studying BLMH's biological functions, particularly its dual role in cancer therapy—both as a determinant of bleomycin resistance and as a potential biomarker for drug efficacy. Additionally, altered BLMH expression has been implicated in neurodegenerative disorders like Alzheimer’s disease, where it may influence amyloid-β peptide processing.
Validated in techniques such as Western blotting, immunohistochemistry, and ELISA, BLMH antibodies aid in elucidating tissue-specific expression patterns and regulatory mechanisms. Their development has advanced research into BLMH's structure-function relationships, substrate specificity, and involvement in pathological conditions, making them essential for both basic and translational studies.
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