| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Radiation-inducible immediate-early gene IEX-1, Differentiation-dependent gene 2 protein, Protein DIF-2, Immediate early protein GLY96, Immediate early response 3 protein, PACAP-responsive gene 1 protein, Protein PRG1, IER3, DIF2, IEX1, PRG1 |
| Entrez GeneID | 8870 |
| WB Predicted band size | 16.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This IER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-65 amino acids from the N-terminal region of human IER3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"IER3 regulates cell proliferation and apoptosis through the NF-κB pathway via its N-terminal domain"**
- **作者**: Zhang L, et al.
- **摘要**: 研究利用N端特异性抗体证实IER3通过其N端结构域调控NF-κB信号通路，影响肿瘤细胞增殖和凋亡。抗体被用于免疫沉淀和Western blot分析，揭示IER3的分子机制。
2. **"Development of a novel monoclonal antibody targeting the N-terminal region of IER3 for stress response studies"**
- **作者**: Tanaka K, et al.
- **摘要**: 描述了针对IER3蛋白N端的单克隆抗体的开发与验证，该抗体成功应用于免疫组化及流式细胞术，证明IER3在氧化应激中通过N端介导细胞保护作用。
3. **"N-terminal phosphorylation of IER3 modulates its pro-apoptotic function in neuronal cells"**
- **作者**: Müller R, et al.
- **摘要**: 通过N端特异性抗体检测IER3的磷酸化状态，揭示其在神经元细胞凋亡中的动态调控，研究显示N端修饰影响与Bcl-2家族的相互作用。
4. **"Antibody-based detection of IER3 isoforms in cardiovascular disease models"**
- **作者**: Gupta S, et al.
- **摘要**: 利用N端抗体区分IER3不同剪切变体，发现其在心肌缺血再灌注损伤中的表达差异，抗体应用于组织样本的免疫荧光及蛋白质互作分析。
(注：上述文献为示例性概括，实际引用需根据真实发表文献调整。)
The IER3 (Immediate Early Response 3) protein, also known as IEX-1 or Gly96. is a stress-inducible regulator involved in apoptosis, cell proliferation, and immune responses. It is encoded by the *IER3* gene, which belongs to the immediate-early gene family activated rapidly by diverse stimuli, including cytokines, radiation, and cellular stress. The IER3 (N-term) antibody specifically targets the N-terminal region of the protein, which contains critical functional domains, such as phosphorylation sites and regions mediating protein-protein interactions. This antibody is commonly used to study IER3 expression, localization, and molecular interactions in various cellular contexts. Researchers employ it in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate IER3's role in stress response pathways, cancer progression, autoimmune disorders, and neurodegenerative diseases. The N-terminal specificity helps distinguish IER3 from potential splice variants or homologous proteins. Validation of this antibody typically includes testing in knockdown/knockout models to confirm specificity, given the protein's low basal expression under non-stressed conditions. Its application has contributed to understanding how IER3 modulates NF-κB signaling, mitochondrial stability, and cell survival decisions, making it a valuable tool in both basic and translational research.
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