Rabbit Polyclonal IER3(N-term) Antibody

The IER3 (Immediate Early Response 3) protein, also known as IEX-1 or Gly96. is a stress-inducible regulator involved in apoptosis, cell proliferation, and immune responses. It is encoded by the *IER3* gene, which belongs to the immediate-early gene family activated rapidly by diverse stimuli, including cytokines, radiation, and cellular stress. The IER3 (N-term) antibody specifically targets the N-terminal region of the protein, which contains critical functional domains, such as phosphorylation sites and regions mediating protein-protein interactions. This antibody is commonly used to study IER3 expression, localization, and molecular interactions in various cellular contexts. Researchers employ it in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate IER3's role in stress response pathways, cancer progression, autoimmune disorders, and neurodegenerative diseases. The N-terminal specificity helps distinguish IER3 from potential splice variants or homologous proteins. Validation of this antibody typically includes testing in knockdown/knockout models to confirm specificity, given the protein's low basal expression under non-stressed conditions. Its application has contributed to understanding how IER3 modulates NF-κB signaling, mitochondrial stability, and cell survival decisions, making it a valuable tool in both basic and translational research.