Rabbit Polyclonal GRB14(N-Term) Antibody

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     Overlay histogram showing A549 cells stained with P34644(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34644, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG,DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed,  0. 1% Triton X-100 permeabilized A549 cells labeling GRB14 with P34644 at 1/25 dilution,  followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green).  Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing A549 cells stained with P34644(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34644,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The GRB14 (N-Term) antibody is a specific tool designed to detect the N-terminal region of Growth Factor Receptor-Bound Protein 14 (GRB14), an adaptor protein involved in receptor tyrosine kinase (RTK) signaling. GRB14 belongs to the GRB7/10/14 family and regulates cellular processes such as proliferation, differentiation, and metabolism by modulating interactions between activated receptors and downstream signaling components. Its N-terminal domain plays a role in binding to phosphorylated tyrosine residues on RTKs, including the insulin receptor, thereby influencing insulin signaling and glucose homeostasis. Dysregulation of GRB14 has been linked to metabolic disorders like type 2 diabetes and certain cancers.

This antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study GRB14 expression, localization, and interactions in various tissues or cell lines. By targeting the N-terminal region, it avoids cross-reactivity with other GRB family members, ensuring specificity. Researchers employ this tool to explore GRB14’s role in insulin resistance, where its overexpression inhibits receptor autophosphorylation, and in cancer progression, where it may modulate RTK-driven pathways like MAPK/ERK. Validated in multiple species, including human and mouse, the GRB14 (N-Term) antibody is essential for elucidating molecular mechanisms in metabolic and oncogenic research.