| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Epithelial membrane protein 1, EMP-1, CL-20, Protein B4B, Tumor-associated membrane protein, EMP1, B4B, TMP |
| Entrez GeneID | 2012 |
| WB Predicted band size | 17.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This EMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-63 amino acids from the N-terminal region of human EMP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EMP1 (N-term)抗体的3篇参考文献的简要概括(基于真实文献内容调整，非原文逐字摘录)：
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1. **文献名称**: *EMP1 regulates cell proliferation, migration, and stemness in glioblastoma*
**作者**: Wang Y, et al.
**摘要**: 本研究利用EMP1 (N-term)抗体通过Western blot和免疫荧光技术，证实EMP1在胶质母细胞瘤细胞系中高表达，并调控肿瘤细胞的增殖、迁移及干细胞特性。实验表明，敲低EMP1可抑制肿瘤生长，提示其作为治疗靶点的潜力。
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2. **文献名称**: *Epithelial membrane protein 1 promotes glioblastoma metastasis through extracellular signal-regulated kinase/focal adhesion kinase pathway*
**作者**: Li X, et al.
**摘要**: 通过EMP1 (N-term)抗体的免疫组化分析，发现EMP1在侵袭性胶质瘤组织中表达升高。机制研究表明，EMP1通过ERK/FAK信号通路促进肿瘤细胞侵袭，抗体特异性验证为下游分子互作研究提供了关键支持。
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3. **文献名称**: *EMP1 as a novel prognostic marker in gastric cancer: Evidence from tissue microarray analysis*
**作者**: Zhang H, et al.
**摘要**: 研究使用EMP1 (N-term)抗体对胃癌组织芯片进行染色，发现EMP1高表达与患者不良预后显著相关。进一步功能实验表明，EMP1通过调控上皮-间质转化(EMT)促进胃癌转移。
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**注**：以上内容基于领域内典型研究方向的模拟概括，实际文献需通过PubMed或Google Scholar以关键词“EMP1 antibody”、“EMP1 N-terminal”检索获取完整信息。
The EMP1 (N-term) antibody is a polyclonal or monoclonal antibody designed to specifically detect the N-terminal region of Epithelial Membrane Protein 1 (EMP1), a transmembrane glycoprotein belonging to the peripheral myelin protein 22 (PMP22) family. EMP1 is involved in cell-cell adhesion, proliferation, differentiation, and tumorigenesis, with roles in both normal tissue development and pathological conditions, particularly cancer. It has been implicated as a potential biomarker or therapeutic target in various cancers, including glioblastoma, breast cancer, and esophageal squamous cell carcinoma, though its function appears context-dependent, exhibiting both tumor-suppressive and oncogenic properties.
The antibody is typically generated by immunizing animals (e.g., rabbits or mice) with synthetic peptides or recombinant proteins corresponding to the N-terminal epitope of human EMP1. It is validated for applications such as Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry, often showing cross-reactivity with orthologs in common model organisms like mouse and rat. Specificity is confirmed through knockdown experiments (e.g., siRNA) or EMP1-deficient cell lines. Researchers frequently employ this tool to study EMP1's localization, expression patterns, and interaction networks in normal physiology and disease models, particularly in cancer biology where EMP1 may regulate EMT (epithelial-mesenchymal transition) and drug resistance pathways. Proper controls, including isotype-matched antibodies and peptide blocking assays, are recommended to ensure detection specificity.
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