| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Wilms tumor protein, WT33, WT1 |
| Entrez GeneID | 7490 |
| WB Predicted band size | 49.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WT1(Center E361)抗体的3篇参考文献示例(注：文献信息为模拟，实际引用需核实)：
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1. **文献名称**: "WT1 Antigen Expression in Hematopoietic Malignancies"
**作者**: Smith A, et al.
**摘要**: 研究使用WT1(Center E361)抗体通过免疫组织化学分析白血病和骨髓增生异常综合征患者的WT1蛋白表达水平，发现其高表达与疾病进展相关，验证了该抗体的特异性及在临床病理诊断中的应用价值。
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2. **文献名称**: "Role of WT1 in Ovarian Carcinoma: Immunohistochemical Analysis"
**作者**: Brown C, et al.
**摘要**: 采用WT1(Center E361)抗体对卵巢癌组织进行染色，发现其在浆液性癌中显著阳性，支持了WT1作为卵巢癌分型标志物的作用，并强调了该抗体在肿瘤鉴别诊断中的可靠性。
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3. **文献名称**: "WT1 Expression in Pediatric Renal Tumors: A Comparative Study"
**作者**: Lee J, et al.
**摘要**: 通过Western blot和免疫组化(使用WT1 Center E361抗体)对比儿童肾母细胞瘤与其他肾肿瘤的WT1表达差异，证实该抗体在区分肾母细胞瘤中的高敏感性和特异性。
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**注意事项**：
- 实际文献需通过PubMed、Google Scholar等平台以关键词“WT1 antibody E361”或“Center E361 WT1”检索。
- 部分厂商(如Cell Signaling Technology)官网可能提供抗体相关引用文献列表。
- 建议结合具体实验目的(如癌种、技术方法)筛选文献。
The WT1 (Wilms tumor 1) antibody, particularly the clone Center E361. is a widely used tool in diagnostic pathology and research to detect the WT1 protein, a transcription factor encoded by the *WT1* gene located on chromosome 11p13. Initially identified for its role in Wilms tumorigenesis, WT1 regulates cellular proliferation, differentiation, and apoptosis, with complex, context-dependent functions as both a tumor suppressor and oncogene. Its expression is critical during embryogenesis, particularly in kidney and gonadal development, but aberrant WT1 expression is observed in various malignancies, including acute leukemias, ovarian carcinomas, and desmoplastic small round cell tumors.
The Center E361 clone specifically recognizes an epitope in the C-terminal region of the WT1 protein, enabling reliable detection in formalin-fixed, paraffin-embedded tissues via immunohistochemistry (IHC). This antibody is valuable for differentiating histologic subtypes (e.g., mesotheliomas vs. adenocarcinomas) and assessing WT1’s prognostic significance in cancers like acute myeloid leukemia. However, interpretation requires caution due to variable expression patterns and splice isoforms. WT1 antibodies, including E361. are also employed in research to study gene regulation mechanisms and therapeutic targeting. Their utility underscores WT1’s dual role in development and disease, making them essential in both clinical diagnostics and molecular pathology.
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