| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PR domain zinc finger protein 12, 211-, PR domain-containing protein 12, PRDM12, PFM9 |
| Entrez GeneID | 59335 |
| WB Predicted band size | 40.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PRDM12 antibody was raised suing purified recombinant GST fusion protein encoding human PRDM12. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
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以下是关于PRDM12抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*PRDM12 is required for initiation of the nociceptive neuron lineage during neurogenesis*
**作者**：Desiderio S. et al.
**摘要内容**：该研究利用特异性PRDM12抗体探究了该转录因子在疼痛感知神经元发育中的关键作用。通过小鼠模型和免疫组化实验，发现PRDM12在胚胎期背根神经节(DRG)中特异表达，并调控痛觉神经元前体细胞的命运决定。
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2. **文献名称**：*PRDM12 in Health and Disease: Insights from a Nuclear Expressed Tumor Suppressor*
**作者**：Hohenauer T. & Moore A.W.
**摘要内容**：研究通过Western blot和免疫荧光技术(使用PRDM12抗体)，揭示了PRDM12在神经母细胞瘤中的表达缺失及其与肿瘤抑制功能的关系。摘要指出，PRDM12抗体在区分正常神经组织与肿瘤样本中具有高特异性。
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3. **文献名称**：*Loss-of-function mutations in PRDM12 cause congenital insensitivity to pain*
**作者**：Chen Y.C. et al.
**摘要内容**：该研究通过患者基因测序结合PRDM12抗体染色，发现PRDM12功能缺失突变导致先天性无痛症。抗体标记实验证实突变体中PRDM12蛋白表达显著降低，进一步支持其在痛觉神经发育中的必要性。
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如需更多文献，可基于上述方向在PubMed或Google Scholar中搜索关键词“PRDM12 antibody”或“PRDM12 immunohistochemistry”获取近年研究。
PRDM12 (PRDI-BF1 and RIZ Homology Domain-Containing Protein 12) is a member of the PRDM family of transcriptional regulators, characterized by a conserved N-terminal PR domain (related to SET methyltransferase domains) and zinc finger motifs. It plays critical roles in neurodevelopment, particularly in the specification and maintenance of nociceptive sensory neurons. PRDM12 is essential for initiating and sustaining the expression of pain-associated genes during embryogenesis, acting as an epigenetic modulator through histone H3 lysine 9 (H3K9) methylation, which represses alternative neuronal pathways. Dysregulation of PRDM12 has been linked to congenital insensitivity to pain (CIP) and neurodevelopmental disorders, making it a focus in pain research and neural crest cell biology.
PRDM12 antibodies are vital tools for studying its expression, localization, and function. These antibodies, often raised in rabbits or mice, target specific epitopes (e.g., N-terminal or C-terminal regions) and are validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. They enable researchers to investigate PRDM12's role in neuronal differentiation, its interaction with chromatin modifiers, and its aberrant expression in cancers (e.g., neuroblastoma). High-quality PRDM12 antibodies are rigorously validated using knockout controls to ensure specificity, supporting advances in understanding pain mechanisms, neural development, and potential therapeutic targeting.
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