| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein C-ets-2, ETS2 |
| Entrez GeneID | 2114 |
| WB Predicted band size | 53.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ETS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-173 amino acids from the Central region of human ETS2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及ETS2抗体应用的文献概览(内容为模拟生成，非真实文献)：
1. **"ETS2 transcription factor modulates oncogenic signaling in colorectal cancer"**
作者：Lee, H. et al.
摘要：研究通过Western blot和免疫组化使用ETS2抗体，证实ETS2在结直肠癌中高表达，并调控Wnt/β-catenin通路促进肿瘤侵袭。
2. **"Immunohistochemical analysis of ETS2 in inflammatory arthritis"**
作者：Martinez, R. et al.
摘要：利用ETS2特异性抗体对关节炎患者滑膜组织染色，发现ETS2表达与炎症因子TNF-α和IL-6水平呈正相关，提示其在慢性炎症中的作用。
3. **"ETS2-dependent regulation of mitochondrial apoptosis in breast cancer"**
作者：Chen, Y. & Wang, X.
摘要：通过ChIP-seq和免疫沉淀实验(使用ETS2抗体)，揭示ETS2通过调控Bcl-2家族基因影响乳腺癌细胞凋亡抵抗的分子机制。
注：以上为基于典型研究场景的模拟案例，实际文献需通过PubMed/Google Scholar检索关键词"ETS2 antibody application"或"ETS2 immunohistochemistry"获取。
The ETS2 antibody is designed to target the ETS2 protein, a member of the ETS (E26 transformation-specific) family of transcription factors. These proteins share a conserved DNA-binding domain (ETS domain) that recognizes purine-rich GGAA/T sequences, enabling regulation of genes involved in cell proliferation, differentiation, apoptosis, and oncogenesis. ETS2. located on human chromosome 21. plays critical roles in embryonic development, particularly in mesodermal and placental formation, and is implicated in tumorigenesis when dysregulated. Overexpression of ETS2 has been linked to cancers like colorectal, breast, and prostate cancer, where it promotes genomic instability, angiogenesis, and metastasis via pathways such as RAS/MAPK and Wnt/β-catenin.
ETS2 antibodies are essential tools in biomedical research, used to detect and quantify ETS2 expression in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple epitopes. Validation often involves knockout cell lines or siRNA-mediated silencing to confirm target specificity. These antibodies aid in studying ETS2's role in cancer progression, developmental biology, and its interaction with co-factors like AP-1 or RUNX1. Clinically, ETS2 expression patterns in tumor tissues, analyzed via IHC, may serve as prognostic biomarkers. Researchers also utilize ETS2 antibodies in ChIP-seq to map DNA-binding sites, elucidating its transcriptional networks. As ETS2 gains attention in therapeutic targeting, its antibodies remain pivotal for mechanistic and translational studies.
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