| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein GLI2, GLI family zinc finger protein 2 {ECO:0000312|HGNC:HGNC:4318}, Tax helper protein, GLI2 (HGNC:4318) |
| Entrez GeneID | 2736 |
| WB Predicted band size | 167.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GLI2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1287-1321 amino acids from the C-terminal region of human GLI2. |
+ +
以下是关于GLI2抗体的3篇参考文献示例(内容为模拟生成，非真实文献)：
---
1. **文献名称**：*"GLI2 expression and Hedgehog signaling in basal cell carcinoma"*
**作者**：Smith A, et al.
**摘要**：研究通过免疫组化(使用GLI2抗体)分析了基底细胞癌中GLI2蛋白的表达水平，证实Hedgehog通路异常激活与GLI2核定位显著相关，为靶向治疗提供依据。
2. **文献名称**：*"Validation of a polyclonal GLI2 antibody for Western blot and immunofluorescence"*
**作者**：Lee J, et al.
**摘要**：系统性验证了某商业GLI2抗体的特异性，通过siRNA敲低实验和过表达模型证明其可特异性识别GLI2蛋白，并优化了实验条件以减少交叉反应。
3. **文献名称**：*"GLI2 regulates neural tube patterning in mouse embryogenesis"*
**作者**：Chen R, et al.
**摘要**：利用GLI2抗体进行胚胎组织染色，发现GLI2在小鼠神经管发育中的梯度表达对背腹轴分化至关重要，并揭示其与Shh信号的动态调控关系。
---
**备注**：以上文献信息为虚构示例，实际引用需通过PubMed/Google Scholar等平台检索关键词(如“GLI2 antibody validation”、“GLI2 immunohistochemistry”)获取真实文献。
The GLI2 antibody is a crucial tool in studying the Hedgehog (Hh) signaling pathway, a key regulator of embryonic development, tissue homeostasis, and cancer. GLI2. a member of the GLI family of zinc-finger transcription factors (GLI1-3), acts as a primary mediator of Hh signaling. Upon pathway activation, GLI2 translocates to the nucleus to regulate the expression of target genes involved in cell proliferation, differentiation, and survival. Unlike GLI1. which functions mainly as an amplifier, GLI2 undergoes complex post-translational modifications that modulate its activity, making it a central focus in developmental biology and oncogenesis research.
GLI2 antibodies are widely used to detect and quantify GLI2 protein levels in various experimental models, including Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate GLI2's role in diseases such as basal cell carcinoma, medulloblastoma, and other cancers driven by Hh pathway dysregulation. Additionally, these antibodies aid in studying developmental disorders like holoprosencephaly, where GLI2 mutations disrupt craniofacial and neural patterning. Researchers also utilize GLI2 antibodies to investigate ciliary signaling, as GLI2 processing occurs in primary cilia. Specificity and validation (e.g., knockout controls) are critical, as GLI proteins share structural homology. Commercial GLI2 antibodies often target distinct regions (e.g., N-terminal or full-length) to differentiate between inactive precursors and active isoforms, providing insights into pathway dynamics and therapeutic targeting.
×
