| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Putative protein arginine N-methyltransferase 9, Putative protein arginine N-methyltransferase 10, 211-, PRMT9, PRMT10 |
| Entrez GeneID | 90826 |
| WB Predicted band size | 94.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PRMT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human PRMT10. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于PRMT10(N-term)抗体的参考文献示例(**注：部分文献信息可能为假设性示例，建议进一步核实**)：
1. **"Identification and Characterization of PRMT10 as a Novel Protein Arginine Methyltransferase"**
*作者：Smith A, et al. (2021)*
**摘要**：本研究首次报道PRMT10的发现，证实其甲基转移酶活性，并通过针对N端结构域的多克隆抗体验证其在哺乳动物细胞中的表达及亚细胞定位。
2. **"PRMT10 Regulates Neural Stem Cell Differentiation via Histone H4 Methylation"**
*作者：Chen L, et al. (2022)*
**摘要**：利用PRMT10 N端特异性抗体进行免疫沉淀和Western blot分析，揭示PRMT10通过H4R3me2修饰调控神经干细胞分化的分子机制。
3. **"A Functional Screen Identifies PRMT10 as a Critical Regulator of DNA Damage Response"**
*作者：Wang Y, et al. (2023)*
**摘要**：通过N端抗体阻断实验，证明PRMT10的N端结构域在DNA损伤修复中起关键作用，并解析其与ATM激酶的相互作用。
4. **"Commercial Antibody Validation for PRMT10: Challenges and Applications"**
*作者：Johnson R, et al. (2020)*
**摘要**：系统评估不同PRMT10抗体(包括N-term抗体)的特异性，强调其在免疫组化和蛋白质组学研究中的适用性及局限性。
**注意**：以上文献名称及内容为模拟场景，实际研究中PRMT10可能尚未被广泛报道(目前已知PRMT家族成员为PRMT1-9)。建议通过PubMed或Google Scholar以“PRMT10 antibody”或“PRMT10 N-terminal”为关键词检索最新文献，并优先选择近3年内的研究。
PRMT10 (Protein Arginine Methyltransferase 10) is a member of the PRMT family, which catalyzes the transfer of methyl groups to arginine residues on target proteins, influencing diverse cellular processes such as transcriptional regulation, RNA processing, and signal transduction. PRMT10. though less characterized compared to other PRMTs (e.g., PRMT1. PRMT5), is classified as a Type I enzyme, mediating asymmetric dimethylation of arginine residues. It is implicated in various biological functions, including neurodevelopment, metabolic regulation, and cell differentiation, though its specific substrates and mechanistic roles remain under active investigation.
The PRMT10 (N-term) antibody is designed to target the N-terminal region of the protein, enabling the detection and study of PRMT10 expression, localization, and interactions. This antibody is critical for applications like Western blotting, immunofluorescence, and immunoprecipitation, aiding in elucidating PRMT10’s molecular functions. Its specificity for the N-terminus helps distinguish PRMT10 from other PRMT family members or splice variants, ensuring accurate experimental results. Validation of the antibody typically involves knockout or knockdown controls to confirm minimal cross-reactivity.
Research utilizing this antibody has begun uncovering PRMT10’s potential roles in diseases, including cancer and neurological disorders, where aberrant arginine methylation may contribute to pathogenesis. Ongoing studies aim to map its substrate profile and regulatory networks, positioning PRMT10 as a promising therapeutic or diagnostic target.
×
