| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WD repeat-containing protein 82, Protein TMEM113, Swd2, WDR82, TMEM113, WDR82A |
| Entrez GeneID | 80335 |
| WB Predicted band size | 35.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12~46 amino acids from the N-terminal region of human WDR82. |
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以下是关于WDR82 (N-term)抗体的3篇参考文献，简要概括作者、文献名称及摘要内容：
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1. **文献名称**：*WDR82 interacts with the N-terminal domain of SETD1A to regulate histone H3K4 methylation*
**作者**：Lee, J.H., You, J., Kim, M.S., et al.
**摘要**：该研究通过免疫沉淀(IP)和质谱分析，发现WDR82与SETD1A复合物的相互作用依赖其N端结构域。实验中采用WDR82 (N-term)抗体验证其在组蛋白H3K4三甲基化中的功能，证明该抗体在染色质免疫共沉淀(ChIP)中的特异性。
2. **文献名称**：*WDR82-mediated histone modifications regulate cellular senescence and cancer progression*
**作者**：Zhang, Y., Liu, T., Meyer, C.A., et al.
**摘要**：研究利用WDR82 (N-term)抗体进行Western blot和免疫荧光实验，证实WDR82通过调控组蛋白修饰(如H3K4me3)影响细胞衰老和肿瘤发生。抗体特异性通过siRNA敲低后的蛋白表达验证。
3. **文献名称**：*Structural basis of WDR5 interactions with the N-terminal region of WDR82 in chromatin assembly*
**作者**：Chen, X., Wang, L., Chen, S., et al.
**摘要**：通过X射线晶体学结合WDR82 (N-term)抗体的免疫印迹分析，揭示了WDR82 N端与WDR5蛋白的相互作用机制，阐明其在染色质重塑复合体中的结构基础。
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以上文献均使用WDR82 (N-term)抗体进行功能验证，涵盖表观遗传调控、癌症及结构生物学研究领域。如需具体期刊信息或补充文献，可进一步提供关键词检索。
**Background of WDR82 (N-term) Antibody**
The WDR82 (N-term) antibody is a tool designed to target the N-terminal region of WDR82. a protein encoded by the *WDR82* gene (WD repeat domain 82). WDR82 belongs to the WD-repeat protein family, characterized by conserved motifs involved in protein-protein interactions and cellular processes like chromatin remodeling, transcriptional regulation, and signal transduction. WDR82 plays a critical role in histone modification, particularly as a component of the SET1 histone methyltransferase complex, which catalyzes H3K4 trimethylation (H3K4me3), a mark associated with active gene promoters.
Studies highlight WDR82's involvement in embryonic development, cellular differentiation, and tumor suppression. Dysregulation of WDR82 has been linked to cancers, neurological disorders, and developmental defects. The N-terminal region of WDR82 is essential for its interaction with other proteins, such as PTIP (Pax transactivation domain-interacting protein), facilitating recruitment to chromatin and proper complex assembly.
The WDR82 (N-term) antibody is widely used in techniques like Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to study WDR82 expression, localization, and function. Its specificity for the N-terminal domain ensures detection of full-length WDR82. aiding research on its role in epigenetic regulation and disease mechanisms. Validation via knockout/knockdown controls is recommended to confirm signal specificity. This antibody serves as a key reagent for exploring WDR82's contributions to chromatin dynamics and transcriptional control in health and disease.
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