Rabbit Polyclonal COL5A1(N-term) Antibody

+ +

•  

   
     Anti-COL5A1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 184 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     Anti-COL5A1 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 184 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     COL5A1 Antibody (N-term) (Cat. #P34604) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).    

•  

   
     Immunohistochemical analysis of paraffin-embedded Human heart tissue using P34604 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature,  antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

•  

   
     Immunohistochemical analysis of paraffin-embedded Human uterus tissue using P34604 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature,  antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

•  

   
     COL5A1 antibody (N-term) (Cat. #P34604) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.  This data demonstrates the use of the COL5A1 antibody (N-term) for immunohistochemistry.  Clinical relevance has not been evaluated.    

The COL5A1 (N-term) antibody is a tool used to detect the N-terminal region of the COL5A1 protein, a critical component of type V collagen. COL5A1 encodes the alpha-1 chain of type V collagen, a fibrillar collagen essential for regulating the assembly and organization of heterotypic collagen fibrils, particularly those containing type I collagen. This collagen plays a structural role in tissues like skin, tendons, and placenta, contributing to their tensile strength and integrity. Mutations in COL5A1 are linked to Ehlers-Danlos syndrome (EDS), a connective tissue disorder characterized by hyperelastic skin, joint hypermobility, and vascular fragility.

The antibody targets the N-terminal propeptide region of COL5A1. which is involved in procollagen processing and fibril formation. Researchers use this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study COL5A1 expression, localization, and post-translational modifications in normal and pathological contexts. Its specificity aids in investigating collagen V’s role in extracellular matrix (ECM) dynamics, tissue development, and diseases such as fibrosis, cancer (e.g., tumor stroma remodeling), and inherited connective tissue disorders. Validation via knockout controls or siRNA knockdown is recommended to confirm signal specificity, as cross-reactivity with related collagens may occur.