Rabbit Polyclonal RAB28 Antibody

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     Anti-RAB28 Antibody (Center) at 1:1000 dilution + Human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing A2058 cells stained with P34601(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min.  The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     Immunohistochemical analysis of paraffin-embedded human kidney tissue using P34601 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

The RAB28 antibody is a crucial tool for studying the RAB28 protein, a member of the RAS-associated binding (RAB) family of small GTPases. RAB28 is involved in intracellular vesicle trafficking, membrane dynamics, and cilia-related functions, particularly in photoreceptor cells and epithelial tissues. It plays a role in maintaining retinal integrity, lipid metabolism, and autophagy regulation. Mutations in the RAB28 gene are linked to cone-rod dystrophy and other ciliopathies, highlighting its importance in ocular health.

RAB28 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies enable detection of endogenous RAB28 via techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Specificity is validated using knockout cell lines or tissues, with many antibodies targeting conserved regions (e.g., the GTP-binding domain or C-terminal epitopes). Researchers use them to explore RAB28's subcellular localization, interaction partners, and dysregulation in disease models.

When selecting a RAB28 antibody, considerations include species reactivity (human, mouse, rat), application compatibility, and post-translational modification recognition (e.g., prenylation). Commercial antibodies often cite validation in peer-reviewed studies, but independent verification is recommended due to variability in epitope accessibility across experimental conditions.