Rabbit Polyclonal SCEL Antibody

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     Anti-SCEL Antibody (Center) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing A431 cells stained with P34599(green line).  The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     Immunohistochemical analysis of paraffin-embedded human skin tissue using P34599 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

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     Immunohistochemical analysis of paraffin-embedded human esophagus  tissue using P34599 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

SCEL antibodies target sciellin (SCEL), a protein encoded by the *SCEL* gene, which is predominantly expressed in stratified squamous epithelia, particularly the epidermis. Sciellin plays a critical role in keratinocyte differentiation and the formation of the cornified envelope, a structure essential for skin barrier function. It interacts with other proteins like loricrin and involucrin during terminal differentiation, contributing to epidermal integrity and resilience.

Research on SCEL antibodies has been driven by their utility in studying skin biology and pathology. These antibodies are employed in techniques such as immunohistochemistry and Western blotting to visualize sciellin expression and localization in tissues. Dysregulation of sciellin has been implicated in dermatological conditions, including psoriasis and eczema, where disrupted cornified envelope assembly compromises barrier function. Additionally, SCEL antibodies aid in exploring genetic disorders linked to *SCEL* mutations, such as some forms of palmoplantar keratoderma.

Beyond basic research, SCEL antibodies hold potential in translational applications. They may serve as biomarkers for diagnosing skin diseases or monitoring therapeutic responses. Recent studies also investigate their role in cancer, as altered sciellin expression has been observed in certain carcinomas. However, the functional significance of these findings remains under exploration. Overall, SCEL antibodies are vital tools for dissecting epidermal biology and its associated disorders.