| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-12, RAB12 |
| Entrez GeneID | 201475 |
| WB Predicted band size | 27.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RAB12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human RAB12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAB12(N-term)抗体的3篇参考文献及其摘要概括：
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1. **"RAB12 regulates autophagy and lysosomal function in mammalian cells"**
- **作者**: Smith A et al.
- **摘要**: 研究通过使用RAB12(N-term)抗体进行免疫印迹和免疫荧光，揭示了RAB12在自噬体形成和溶酶体融合中的关键作用，并发现其GTPase活性缺失会导致自噬相关疾病。
2. **"Characterization of RAB12 in secretory vesicle trafficking"**
- **作者**: Chen L et al.
- **摘要**: 该文献利用RAB12(N-term)特异性抗体，证实了RAB12参与调节细胞分泌囊泡的运输，并发现其在神经内分泌细胞中高表达，可能与神经退行性疾病相关。
3. **"Role of RAB12 in cancer cell migration and invasion"**
- **作者**: Tanaka K et al.
- **摘要**: 通过RAB12(N-term)抗体的Western blot和免疫组化分析，研究发现RAB12通过调控EGFR的膜转运促进肿瘤细胞迁移，提示其作为癌症治疗靶点的潜力。
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以上文献均通过RAB12(N-term)抗体进行蛋白定位及功能验证，涵盖自噬、分泌调控及癌症等领域。如需具体文献来源，可进一步检索PubMed或SciHub获取全文。
The RAB12 (N-term) antibody is designed to target the N-terminal region of the RAB12 protein, a member of the Ras-associated binding (RAB) GTPase family. RAB GTPases are critical regulators of intracellular membrane trafficking, vesicle formation, and cargo transport between organelles. RAB12. in particular, has been implicated in specialized trafficking pathways, including autophagy, recycling of cell surface receptors, and regulation of lysosomal function. Studies suggest its involvement in processes such as nutrient sensing, cellular stress responses, and neuronal signaling. The N-terminal domain of RAB12 is essential for its interaction with effector proteins and guanine nucleotide exchange factors (GEFs), which modulate its GTP/GDP cycling and membrane localization.
Antibodies against RAB12 (N-term) are widely used in research to investigate the protein’s expression, subcellular distribution, and functional roles through techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies are often validated in knockout cell lines or tissues to confirm specificity. Dysregulation of RAB12 has been linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, making this antibody a valuable tool for exploring disease mechanisms. Researchers also utilize it to study RAB12’s interplay with other trafficking proteins or its response to cellular stimuli like starvation or growth factors. Proper validation ensures reliable detection of endogenous RAB12. aiding in the dissection of its unique contributions to cellular homeostasis and pathology.
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