Rabbit Polyclonal AP1M1 Antibody

The AP1M1 antibody targets the μ1 subunit of the adaptor protein complex 1 (AP-1), a key player in intracellular membrane trafficking. AP-1. composed of β, γ, μ, and σ subunits, facilitates vesicle formation and cargo sorting between the trans-Golgi network (TGN), endosomes, and lysosomes. The μ1 subunit (AP1M1) specifically recognizes tyrosine-based sorting signals on transmembrane proteins, enabling selective packaging into clathrin-coated vesicles. This process is critical for maintaining cellular homeostasis, receptor recycling, and polarized protein distribution in epithelial cells. Dysregulation of AP1M1 has been linked to genetic disorders like MEDNIK syndrome (characterized by neurodevelopmental defects and ichthyosis) and cancers, where altered membrane trafficking contributes to tumor progression. Researchers use AP1M1 antibodies in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study its expression, subcellular localization, and interactions. These studies help elucidate mechanisms underlying AP-1-mediated trafficking defects in diseases. The antibody’s specificity for AP1M1 across species (e.g., human, mouse) supports cross-disciplinary research, enabling comparative analyses in model organisms. Its applications extend to diagnosing trafficking-related pathologies and screening therapeutic targets.