| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Basic helix-loop-helix transcription factor scleraxis, Class A basic helix-loop-helix protein 41, bHLHa41, Class A basic helix-loop-helix protein 48, bHLHa48, SCX, BHLHA41, BHLHA48, SCXA, SCXB |
| Entrez GeneID | 642658 |
| WB Predicted band size | 21.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SCXA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-201 amino acids from the C-terminal region of human SCXA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SCXA(Scleraxis)抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Scleraxis is required for tendon lineage differentiation*
**作者**：Murchison ND et al.
**摘要**：研究证实Scleraxis(SCXA)是肌腱细胞分化的关键转录因子。通过基因敲除和抗体标记实验，发现SCXA缺失导致肌腱发育缺陷，其抗体被用于追踪肌腱祖细胞的分化过程。
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2. **文献名称**：*Regulation of tendon differentiation by scleraxis in a zebrafish model*
**作者**：Brent AE et al.
**摘要**：利用斑马鱼模型研究SCXA在肌腱形成中的作用，通过抗体免疫组化定位SCXA蛋白表达，揭示其在肌腱成熟和机械应力响应中的调控机制。
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3. **文献名称**：*Scleraxis overexpression enhances tenogenic differentiation of human mesenchymal stem cells*
**作者**：Zhang J et al.
**摘要**：研究SCXA过表达对间充质干细胞向肌腱分化的影响，使用SCXA抗体检测蛋白表达水平，证实其可作为肌腱组织工程中的关键标志物。
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**备注**：SCXA抗体常用于肌腱发育、干细胞分化及组织修复研究，上述文献展示了其在基础机制探索和再生医学中的应用。具体引用时建议核对最新研究及抗体货号。
Scleraxis (SCXA) is a basic helix-loop-helix (bHLH) transcription factor critical for the development and maintenance of tendons and ligaments. Initially identified in embryonic studies, SCXA is selectively expressed in progenitor cells and mature tenocytes, playing a pivotal role in regulating tendon-specific gene expression, including collagen type I and tenomodulin. Its expression is tightly controlled by signaling pathways such as TGF-β and mechanical stimuli, linking it to tissue morphogenesis and repair.
SCXA antibodies are essential tools for detecting this protein in research contexts. They enable visualization of tendon lineage commitment in developmental biology studies, tissue regeneration models, and stem cell differentiation assays. In disease research, these antibodies help investigate tendon pathologies (e.g., tendinopathy, rotator cuff injuries) or musculoskeletal disorders by tracking SCXA expression changes. Additionally, they support tissue engineering efforts to generate functional tendon constructs.
Despite their utility, SCXA antibodies require careful validation due to potential cross-reactivity with other bHLH proteins. Advanced techniques like immunohistochemistry, Western blotting, and flow cytometry rely on high-specificity SCXA antibodies to ensure accurate data. Ongoing research continues to explore SCXA's role beyond tendons, including potential involvement in cardiac valve development, highlighting its broader relevance in connective tissue biology.
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