| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23 |
| Entrez GeneID | 27005 |
| WB Predicted band size | 62.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-436 amino acids from the C-terminal region of human USP21. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于USP21抗体的示例性参考文献(请注意，文献信息为模拟示例，实际引用需核实真实来源)：
1. **"USP21 deubiquitinates and stabilizes RIP2 to promote inflammatory signaling"**
- **作者**: Li, X. et al.
- **摘要**: 本研究揭示USP21通过去泛素化RIP2蛋白增强NF-κB信号通路，促进炎症反应。实验使用特异性USP21抗体进行免疫共沉淀和Western blot验证其相互作用。
2. **"Development of a monoclonal antibody against USP21 for functional characterization in cancer models"**
- **作者**: Wang, Y. et al.
- **摘要**: 报道一种高特异性USP21单克隆抗体的开发，验证其在免疫组化(IHC)和流式细胞术中的应用，并证明USP21在结直肠癌中高表达且与预后相关。
3. **"USP21 regulates cell cycle progression by stabilizing Cyclin D1"**
- **作者**: Chen, J. et al.
- **摘要**: 发现USP21通过去泛素化Cyclin D1调控细胞周期进程。研究利用CRISPR敲除和USP21抗体进行染色质免疫沉淀(ChIP)，揭示其在G1/S转换中的作用。
4. **"USP21 modulates antiviral immune response via deubiquitinating STING"**
- **作者**: Zhang, H. et al.
- **摘要**: 阐明USP21通过去泛素化STING蛋白抑制I型干扰素信号通路，实验依赖USP21抗体进行免疫荧光和蛋白质稳定性分析。
**建议**：实际文献检索可通过PubMed或Google Scholar以关键词“USP21 antibody”、“USP21 function”等查找最新研究。
The USP21 antibody is a research tool designed to detect and study ubiquitin-specific protease 21 (USP21), a member of the deubiquitinating enzyme (DUB) family. USP21 plays a critical role in regulating protein stability and function by removing ubiquitin chains from target substrates, thereby influencing cellular processes such as DNA repair, immune response, and apoptosis. It is implicated in modulating key signaling pathways, including NF-κB and Wnt/β-catenin, and interacts with substrates like RIPK1 and β-catenin to fine-tune their activity. Dysregulation of USP21 has been linked to diseases such as cancer, neurodegenerative disorders, and inflammatory conditions, making it a potential therapeutic target.
The USP21 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate USP21 expression, localization, and interactions in various biological contexts. Its specificity and sensitivity are crucial for distinguishing USP21 from other DUBs due to structural similarities within the enzyme family. Recent studies have highlighted USP21's role in tumor progression, particularly in promoting cancer cell survival and chemoresistance, driving demand for reliable antibodies to explore its mechanisms. Validated USP21 antibodies are essential for advancing research into its physiological and pathological functions, as well as for developing targeted therapies.
×
