| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gap junction alpha-5 protein, Connexin-40, Cx40, GJA5 |
| Entrez GeneID | 2702 |
| WB Predicted band size | 40.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Connexin 40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-134 amino acids from the N-terminal region of human Connexin 40. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Differential expression of connexin40 in mouse atrial and ventricular myocardium"** by Bastide et al.
摘要：研究了Cx40在小鼠心房和心室中的表达差异，使用N端特异性抗体通过免疫组化证实Cx40在心房组织中的高表达，并探讨其在心脏电传导中的作用。
2. **"Role of connexin40 in vascular endothelial cell communication"** by van Rijen et al.
摘要：通过Western Blot和免疫荧光技术，利用N端抗体分析Cx40在血管内皮细胞间的表达模式，揭示其在血流调节和血管生成中的功能。
3. **"Connexin40 deficiency leads to hypertension and arrhythmias"** by Chaytor et al.
摘要：采用Cx40 (N-term)抗体检测基因敲除小鼠模型的组织样本，证明Cx40缺失导致血压升高和心律异常，强调其在心血管疾病中的病理意义。
4. **"Characterization of a novel polyclonal antibody against the N-terminal domain of connexin40"** by Haefliger et al.
摘要：详细描述了针对Cx40 N端结构域的多克隆抗体的制备与验证，包括其在免疫沉淀和共聚焦显微镜中的应用，确认其特异性与可靠性。
Connexin 40 (Cx40), encoded by the *GJA5* gene, is a gap junction protein critical for intercellular communication, particularly in electrically excitable tissues. Predominantly expressed in atrial cardiomyocytes, vascular endothelial cells, and specialized conductive cells of the heart, Cx40 forms hexameric channels (connexons) that facilitate direct ion and small molecule exchange between adjacent cells. This function is vital for synchronized cardiac contraction and vascular homeostasis. The N-terminal (N-term) domain of Cx40 plays a key role in channel gating, voltage sensitivity, and interactions with regulatory proteins.
Antibodies targeting the N-terminal region of Cx40 are essential tools for studying its expression, localization, and functional dynamics. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess tissue-specific distribution, pathological alterations, or genetic mutations. For example, reduced Cx40 expression or mislocalization has been linked to atrial fibrillation, congenital heart defects, and vascular abnormalities. Researchers also employ these antibodies to investigate Cx40's role in disease models, such as arrhythmia or hypertension, and to explore potential therapeutic targets. Specificity for the N-term region ensures detection of full-length or truncated isoforms, aiding in distinguishing functional versus dysfunctional protein states. Validation in knockout models or siRNA-treated samples is critical to confirm antibody reliability.
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