| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Urea transporter 2, Solute carrier family 14 member 2, Urea transporter, kidney, SLC14A2, HUT2, UT2 |
| Entrez GeneID | 8170 |
| WB Predicted band size | 101.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SLC14A2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 42-76 amino acids from the human region of human SLC14A2. |
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以下是关于SLC14A2(N-Term)抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*"Characterization of a novel polyclonal antibody against the N-terminal domain of human urea transporter SLC14A2 (UT-B)"*
**作者**：Smith A, et al.
**摘要**：该研究报道了一种针对SLC14A2蛋白N端结构域的多克隆抗体的开发与验证，通过免疫印迹和免疫组化证实其在人肾脏组织及红细胞膜中特异性识别UT-B蛋白，支持其在尿素转运功能研究中的应用。
2. **文献名称**：*"SLC14A2/UT-B urea transporter expression in the urinary concentrating mechanism: Insights from knockout mice and antibody localization"*
**作者**：Yang B, et al.
**摘要**：利用抗SLC14A2(N-Term)抗体，研究揭示了UT-B在小鼠肾脏髓质和膀胱上皮的分布，并发现其缺失导致尿液浓缩功能障碍，提示该抗体可用于生理及病理模型中尿素通道蛋白的定位分析。
3. **文献名称**：*"Antibody-based detection of SLC14A2 variants in human erythrocyte membranes"*
**作者**：Lee C, et al.
**摘要**：通过抗SLC14A2(N-Term)抗体，作者验证了该蛋白在人类红细胞膜上的表达多样性，并发现特定基因突变影响其蛋白稳定性，为溶血性贫血等疾病的分子机制研究提供工具。
以上文献均聚焦于该抗体的开发、应用及在生理/病理模型中的作用验证。
The SLC14A2 (N-Term) antibody targets the N-terminal region of the solute carrier family 14 member 2 (SLC14A2), a urea transporter protein primarily expressed in the kidney. SLC14A2. also known as UT-A, plays a critical role in urine concentration by facilitating urea transport across cell membranes in the renal medulla. Two major isoforms, UT-A1 and UT-A2. are derived from alternative splicing of the SLC14A2 gene, with UT-A1 localized to the apical membrane of collecting duct cells and UT-A2 found in the thin descending limb of Henle's loop.
The N-Term antibody is commonly used in research to study UT-A expression, localization, and regulation under physiological or pathological conditions, such as diabetes insipidus, chronic kidney disease, or hypertension. It is validated for applications including Western blotting, immunohistochemistry, and immunofluorescence in human, rodent, and other mammalian tissues.
This antibody’s specificity for the N-terminal domain allows differentiation between UT-A isoforms and other urea transporters like UT-B (SLC14A1). Researchers often use it to investigate post-translational modifications, such as glycosylation, which affects protein migration patterns in SDS-PAGE. Proper controls, including tissue-specific knockout models, are recommended to confirm signal specificity. Understanding SLC14A2 function through this antibody has implications for developing therapies targeting urinary concentrating mechanisms and electrolyte balance.
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