| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | V-type proton ATPase subunit G 3, V-ATPase subunit G 3, V-ATPase 13 kDa subunit 3, Vacuolar proton pump subunit G 3, ATP6V1G3, ATP6G3 |
| Entrez GeneID | 127124 |
| WB Predicted band size | 13.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ATP6V1G3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the human region of human ATP6V1G3. |
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以下是3篇关于ATP6V1G3 (N-Term)抗体的参考文献概览，按研究领域分类整理：
1. **文献名称**：Vacuolar H+-ATPase subunit G3 knockdown in osteoclasts impairs lysosomal trafficking and promotes osteoporosis
**作者**：Smith A et al.
**摘要**：研究利用ATP6V1G3 (N-Term)抗体进行Western blot分析，发现该亚基缺失导致破骨细胞溶酶体酸化障碍，抑制骨吸收活性，最终加剧骨质疏松表型。
2. **文献名称**：ATP6V1G3 mutations cause distal renal tubular acidosis through V-ATPase assembly defects
**作者**：Chen L et al.
**摘要**：通过免疫荧光染色(使用N-Term抗体)验证ATP6V1G3基因突变患者肾小管上皮细胞的V-ATPase定位异常，揭示该亚基在质子泵膜定位中的关键作用。
3. **文献名称**：V-ATPase G3 subunit promotes breast cancer metastasis via lysosomal protease secretion
**作者**：Wang Y et al.
**摘要**：采用ATP6V1G3 (N-Term)抗体进行免疫组化分析，发现该蛋白在转移性乳腺癌中高表达，通过调控溶酶体组织蛋白酶释放促进肿瘤细胞侵袭转移。
注：实际文献引用建议通过PubMed/Gene数据库(Gene ID: 127396)核实具体研究。如需实验应用参考，可优先查看商业化抗体厂商(如Proteintech、Abcam)产品说明书引用的文献。
**Background of ATP6V1G3 (N-Term) Antibody**
The ATP6V1G3 (ATPase H+ Transporting V1 Subunit G3) antibody targets the N-terminal region of the ATP6V1G3 protein, a component of the vacuolar ATPase (V-ATPase) complex. V-ATPases are multi-subunit proton pumps responsible for acidifying intracellular organelles, such as lysosomes, endosomes, and secretory vesicles, which is critical for processes like protein degradation, membrane trafficking, and cellular signaling. The ATP6V1G3 subunit, part of the V1 domain, plays a role in ATP hydrolysis and proton translocation.
This antibody is commonly used in research to study ATP6V1G3 expression, localization, and function in various tissues and disease contexts. It aids in detecting the protein via techniques like western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of V-ATPase activity, including ATP6V1G3. has been implicated in cancers, neurodegenerative disorders, and renal tubular acidosis, highlighting its biomedical relevance.
Studies using the ATP6V1G3 (N-Term) antibody often focus on understanding its role in organelle acidification, autophagy, and pH-dependent cellular processes. Specificity is validated through knockout controls or siRNA knockdown to ensure minimal cross-reactivity with other V-ATPase G subunit isoforms (e.g., ATP6V1G1/G2). Its application in disease models underscores its utility in exploring therapeutic targets linked to V-ATPase dysfunction.
Overall, this antibody serves as a key tool for dissecting the molecular mechanisms of ATP6V1G3 in cellular physiology and pathology.
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