Rabbit Polyclonal FAM3A Antibody

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     Anti-FAM3A Antibody (C-Term) at 1:2000 dilution + Human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing A431 cells stained with P34536(green line).  The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P34536 staining FAM3A in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

FAM3A (family with sequence similarity 3 member A) is a protein-coding gene belonging to the FAM3 family, which comprises four members (FAM3A-D) characterized by conserved sequence motifs and putative cytokine-like structures. Although its exact physiological role remains under investigation, FAM3A is implicated in metabolic regulation, particularly in energy homeostasis and insulin secretion. Studies suggest its involvement in mitochondrial function, where it may modulate ATP production in pancreatic β-cells, influencing glucose-stimulated insulin release. Dysregulation of FAM3A has been linked to metabolic disorders, including type 2 diabetes, and certain cancers, where it may act as either a tumor suppressor or promoter depending on tissue context.

FAM3A antibodies are essential tools for detecting and characterizing the expression, localization, and functional mechanisms of FAM3A in both physiological and pathological conditions. These antibodies are widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore FAM3A's tissue distribution, subcellular localization (notably in mitochondria and endoplasmic reticulum), and interaction networks. Recent research also highlights its potential role in neuroprotection and neurodegenerative diseases, expanding its relevance beyond metabolic contexts. Despite progress, further studies are needed to clarify its signaling pathways and therapeutic potential. FAM3A antibodies thus serve as critical reagents in bridging molecular insights to disease-related applications.