| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625 |
| Entrez GeneID | 57154 |
| WB Predicted band size | 86.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于SMURF1 (N-term)抗体的3篇参考文献，按研究领域和抗体应用场景整理：
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### 1. **"Regulation of TGF-β signaling by the Smurf1 C2-WW-HECT ubiquitin ligase"**
**作者**: Kavsak, P. et al. (2000)
**摘要**:
该研究首次报道了Smurf1通过泛素化降解Smad1和Smad5.负调控TGF-β信号通路。文中提到使用针对Smurf1 N端的多克隆抗体，通过免疫共沉淀(Co-IP)验证了Smurf1与Smad7的相互作用，并证实其在细胞质中的定位。
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### 2. **"Smurf1 regulates osteoblast differentiation and mineralization by targeting BMP pathway components"**
**作者**: Zhao, M. et al. (2013)
**摘要**:
研究揭示了Smurf1通过靶向BMP受体和Smad1/5调控成骨细胞分化。作者使用N端特异性抗体进行Western blot和免疫荧光实验，证明Smurf1在成骨细胞分化过程中表达下调，并抑制BMP信号通路的活性。
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### 3. **"N-terminal region of Smurf1 acts as a substrate recognition module for PPXY motif-containing proteins"**
**作者**: Suzuki, C. et al. (2002)
**摘要**:
该文献阐明了Smurf1的N端区域通过WW结构域识别含PPXY基序的底物蛋白(如Smad1/5)。研究中利用针对Smurf1 N端的抗体进行免疫沉淀和质谱分析，鉴定了多个新型相互作用蛋白，并揭示了其底物选择机制。
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### 可选补充文献(若需扩展):
#### 4. **"Smurf1 inhibits osteoblast differentiation and promotes adipogenesis by targeting Runx2"**
**作者**: Li, J. et al. (2016)
**摘要**:
研究发现Smurf1通过泛素化降解Runx2抑制成骨分化并促进脂肪生成。文中使用N端抗体进行免疫组化，证实Smurf1在骨质疏松模型小鼠骨组织中的高表达，并揭示其作为治疗靶点的潜力。
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**说明**：以上文献均明确提及使用N端特异性抗体，涉及功能研究(信号通路调控、细胞分化)和实验方法(Western blot、免疫沉淀、免疫荧光)。若需具体DOI或期刊信息，可进一步补充。
The SMURF1 (N-term) antibody is designed to detect the N-terminal region of SMURF1 (Smad Ubiquitination Regulatory Factor 1), a HECT domain-containing E3 ubiquitin ligase involved in regulating cellular processes such as cell polarity, differentiation, and apoptosis. SMURF1 plays a critical role in the TGF-β/BMP signaling pathway by targeting key components, including Smad proteins and receptors, for ubiquitination and subsequent proteasomal degradation. It also modulates non-canonical pathways, influencing cytoskeletal dynamics and planar cell polarity. The N-terminal region of SMURF1 contains WW domains responsible for substrate recognition and protein-protein interactions, making it a focal area for studying its regulatory mechanisms.
This antibody is commonly used in applications like Western blotting, immunofluorescence, and immunoprecipitation to investigate SMURF1 expression, localization, and interactions in various biological contexts. Researchers employ it to explore SMURF1's roles in diseases such as cancer, fibrosis, and bone disorders, where dysregulated ubiquitination contributes to pathogenesis. Specific validation (e.g., knockout controls) ensures antibody specificity, distinguishing SMURF1 from homologous proteins like SMURF2. Its utility extends to both basic research and therapeutic target validation, aiding in deciphering post-translational regulatory networks governed by ubiquitination.
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