Rabbit Polyclonal CRTR1(N-term) Antibody

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     All lanes : Anti-CRTRT1 Antibody (N-term) at 1:2000 dilution Lane 1: Human kidney lysate Lane 2: Human placenta lysate Lane 3: Mouse kidney lysate Lane 4: Rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed,  0. 1% Triton X-100 permeabilized U-2OS cells labeling TFCP2L1 with P34531 at 1/25 dilution,  followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green).  Immunofluorescence image showing nucleus staining on U-2OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing U-2 OS cells stained with P34531(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34531,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     Immunohistochemical analysis of paraffin-embedded human testis tissue using P34531 performed on the Leica® BOND RXm.  Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.   A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

The CRTR1 (N-term) antibody targets the N-terminal region of Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5. COMP is a multidomain glycoprotein primarily expressed in cartilage, tendons, and ligaments, where it plays a critical role in extracellular matrix organization, chondrocyte proliferation, and collagen fibrillogenesis. It belongs to the thrombospondin family and contains conserved domains, including a pentameric N-terminal domain, epidermal growth factor (EGF)-like repeats, and a C-terminal globular domain. Mutations in the COMP gene are linked to skeletal dysplasias, such as pseudoachondroplasia and multiple epiphyseal dysplasia.

The CRTR1 (N-term) antibody is designed to detect the N-terminal region of COMP, which mediates interactions with collagen and other matrix components. This antibody is widely used in research to study COMP expression, localization, and pathogenic mechanisms in musculoskeletal disorders. It is valuable in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess COMP levels in tissues or cell models, particularly in studies exploring cartilage degeneration, osteoarthritis, or genetic mutations affecting COMP stability. By targeting the N-terminal region, the antibody helps differentiate full-length COMP from degradation products, aiding in the characterization of disease-associated molecular changes. Its specificity makes it a key tool for understanding COMP’s role in development, homeostasis, and disease.