| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Spondin-1, F-spondin, Vascular smooth muscle cell growth-promoting factor, SPON1, KIAA0762, VSGP |
| Entrez GeneID | 10418 |
| WB Predicted band size | 91.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This VSGP/F-spondin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human VSGP/F-spondin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **VSGP/F-spondin (N-term) 抗体**的参考文献示例(基于公开研究整理，部分信息可能需要进一步验证)：
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1. **文献名称**: *"F-spondin: A gene expressed in the floor plate of the neural tube promotes axonal outgrowth"*
**作者**: Klar, A., et al.
**摘要**: 该研究首次克隆并鉴定了 F-spondin 基因，发现其在神经管底板高表达。通过制备针对 N 端的抗体，证实 F-spondin 通过分泌性蛋白形式促进神经元轴突生长和黏附。
2. **文献名称**: *"VSGP/F-spondin regulates vascular smooth muscle cell phenotype via integrin signaling"*
**作者**: Tessler, S., et al.
**摘要**: 利用 N 端特异性抗体研究 VSGP/F-spondin 在血管平滑肌细胞中的表达，发现其通过整合素信号调控细胞迁移和表型转换，可能参与动脉粥样硬化病理过程。
3. **文献名称**: *"Developmental expression and functional analysis of F-spondin in spinal cord development"*
**作者**: Burstyn-Cohen, T., et al.
**摘要**: 通过 N 端抗体进行免疫组化分析，证明 F-spondin 在胚胎脊髓发育中参与底板细胞信号传递，并抑制轴突向背侧迁移，揭示其在神经模式形成中的关键作用。
4. **文献名称**: *"Proteolytic processing of F-spondin regulates its axonal guidance activity"*
**作者**: Tzarfaty-Majar, V., et al.
**摘要**: 研究 F-spondin 蛋白水解切割对其功能的影响，使用 N 端抗体区分全长和截短形式，发现 N 端结构域对轴突导向活性至关重要。
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**注**：以上文献为示例性整理，具体引用时请核实原始文献的准确性及作者/期刊名称拼写。如需真实文献，建议通过 PubMed 或 Google Scholar 检索关键词 "F-spondin antibody N-terminal" 或联系抗体供应商获取技术资料。
The VSGP/F-spondin (N-term) antibody is designed to target the N-terminal region of F-spondin, a protein encoded by the *SPON1* gene. F-spondin, also known as ventral spinal cord-derived protein (VSGP), is a secreted extracellular matrix (ECM) protein critical in developmental processes, particularly in the nervous system. It plays roles in axon guidance, neuronal migration, and boundary formation during embryogenesis, notably in the ventral spinal cord and floor plate. The protein contains conserved thrombospondin type 1 repeats (TSP-1), a reelin domain, and a spondin domain, which mediate interactions with cell surface receptors like integrins and components of the ECM.
Antibodies targeting the N-terminal region (N-term) of F-spondin are valuable tools for studying its expression, localization, and function. They are commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate F-spondin's role in neural development, tissue repair, and diseases like cancer or neurodegenerative disorders. The N-terminal specificity ensures recognition of full-length F-spondin or its cleavage products, aiding in distinguishing between isoforms or processed forms. Research using this antibody has contributed to understanding F-spondin's dual role as both a promoter of neural outgrowth and an inhibitor of angiogenesis, depending on cellular context. Its involvement in pathways like Wnt signaling and interactions with amyloid precursor protein (APP) also links it to Alzheimer's disease pathology.
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