| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tubulin gamma-2 chain, Gamma-2-tubulin, Tubg2 |
| Entrez GeneID | 103768 |
| WB Predicted band size | 51.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This gamma Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 400-434 amino acids from the mouse region of mouse gamma Tubulin. |
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以下是3篇关于γ-Tubulin抗体的代表性文献，简要整理如下：
1. **文献名称**：*Gamma-tubulin is a highly conserved component of the centrosome*
**作者**：Stearns, T., Evans, L., & Kirschner, M. (1991)
**摘要**：通过哺乳动物细胞研究，利用γ-Tubulin抗体结合免疫荧光技术，揭示了γ-Tubulin在中心体的定位，并证明其参与微管成核过程，对细胞分裂中纺锤体形成至关重要。
2. **文献名称**：*γ-Tubulin is required for the structure and function of the microtubule organizing centre in Drosophila neuroblasts*
**作者**：Moritz, M., Braunfeld, M.B., & Alberts, B.M. (1995)
**摘要**：利用果蝇胚胎模型，通过γ-Tubulin抗体的免疫电镜分析，证实γ-Tubulin是中心体核心组分，其缺失导致微管组织紊乱，直接影响细胞极性与分裂。
3. **文献名称**：*Localization of gamma-tubulin in interphase and mitotic cells of the fission yeast Schizosaccharomyces pombe*
**作者**：Horio, T., & Oakley, B.R. (1994)
**摘要**：在裂殖酵母中，通过特异性γ-Tubulin抗体的免疫定位，发现γ-Tubulin富集于纺锤体极体和间期微管组织中心，支持其在微管组装中的保守功能。
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**备注**：以上文献均为早期经典研究，建议结合近年研究(如超分辨率显微技术应用)补充抗体在新型实验场景中的使用案例。
Gamma tubulin is a highly conserved member of the tubulin protein family, primarily known for its critical role in microtubule nucleation and organization. Unlike alpha- and beta-tubulins, which form microtubule polymers, gamma tubulin functions as a core component of the gamma-tubulin ring complex (γ-TuRC). This complex serves as a template for microtubule assembly, anchoring at microtubule-organizing centers (MTOCs) such as centrosomes in animal cells or spindle pole bodies in fungi. By initiating microtubule formation, gamma tubulin ensures proper mitotic spindle assembly, chromosome segregation, and overall cell cycle progression.
Antibodies targeting gamma tubulin are essential tools in cell biology research. They enable visualization and quantification of gamma tubulin localization, particularly in MTOCs, using techniques like immunofluorescence, immunoblotting, or immunoprecipitation. These antibodies help study cellular processes like centrosome maturation, mitotic defects, or ciliopathies. Many commercially available gamma tubulin antibodies are raised against conserved epitopes, allowing cross-reactivity across species (e.g., human, mouse, rat). Specific monoclonal antibodies, such as GTU-88. are widely validated for their specificity to gamma tubulin without cross-reacting with other tubulin isoforms. Researchers frequently use these antibodies as markers for centrosomes or to investigate dysregulation in cancers and neurodegenerative diseases linked to microtubule instability. Proper validation controls remain crucial due to potential off-target signals in certain experimental conditions.
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