Rabbit Polyclonal betaIITubulin Antibody

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     All lanes : Anti-beta II Tubulin Antibody at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Mouse brain lysate Lane 4: Human brain lysate Lane 5: Hela whole cell lysate Lane 6: A431 whole cell lysate Lane 7: 293 whole cell lysate Lane 8: MCF-7 whole cell lysate Lane 9: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized  C2C12 (mouse myoblast cell line) cells labeling beta II Tubulin with P34526 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on C2C12 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing C2C12 cells stained with P34526(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min.  The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

Beta II Tubulin (βII-Tubulin), encoded by the *TUBB2B* gene, is a member of the β-tubulin family, essential for microtubule formation and cytoskeletal dynamics. Microtubules are critical for cell division, intracellular transport, and maintaining cell shape. Among β-tubulin isoforms, βII-Tubulin is widely expressed in mammalian tissues, particularly enriched in neurons and the central nervous system, though not neuron-specific. It plays roles in neuronal development, axon guidance, and synaptic plasticity.

Beta II Tubulin antibodies are vital tools in neuroscience and cell biology research. They specifically recognize the βII isoform, distinguishing it from other β-tubulin subtypes (e.g., βIII in mature neurons or βI in proliferating cells). These antibodies are widely used in techniques like immunohistochemistry, immunofluorescence, and Western blotting to study neuronal differentiation, microtubule organization, and cellular responses to microtubule-targeting drugs (e.g., taxanes or vinca alkaloids). In disease research, βII-Tubulin dysregulation has been linked to neurodevelopmental disorders, cancer (due to altered microtubule stability affecting chemotherapy responses), and ciliopathies. Commercial antibodies are often raised against conserved epitopes, such as the C-terminal region, ensuring cross-species reactivity. Researchers must validate specificity using isoform-specific controls, as cross-reactivity with other β-tubulins can occur. Overall, βII-Tubulin antibodies remain indispensable for probing microtubule-related mechanisms in health and disease.