Rabbit Polyclonal GCA(N-Term) Antibody

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     All lanes : Anti-GCA Antibody (N-Term) at 1:2000 dilution Lane 1: Human cerebellum lysate Lane 2: Human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling GCA with P34525 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing U-2 OS cells stained with P34525(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34525,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The GCA (N-Term) antibody targets the N-terminal region of Grancalcin (GCA), a calcium-binding protein belonging to the EF-hand family. GCA is primarily expressed in immune cells, particularly neutrophils and macrophages, and plays roles in cell adhesion, migration, and inflammatory responses. It interacts with components of the extracellular matrix and intracellular signaling pathways, modulating cytoskeletal dynamics and immune cell functions. The N-terminal region of GCA is critical for its calcium-dependent conformational changes and interactions with binding partners.

Antibodies against GCA (N-Term) are widely used in research to investigate its expression, localization, and mechanistic roles in physiological and pathological contexts. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies leveraging these antibodies have linked GCA to inflammatory diseases, autoimmune disorders, and cancer progression, highlighting its potential as a therapeutic or diagnostic target. Additionally, GCA antibodies aid in exploring calcium signaling pathways and immune cell behavior in experimental models. Their specificity for the N-terminal epitope ensures precise targeting, distinguishing GCA from other EF-hand proteins. Overall, GCA (N-Term) antibodies are vital tools for unraveling the protein's contributions to cellular processes and disease mechanisms.