Rabbit Polyclonal COL14A1 Antibody

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     Anti-COL14A1 Antibody (Center) at 1:2000 dilution + Human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 194 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing Hela cells stained with P34514(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34514,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P34514 staining COL14A1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

COL14A1 antibody targets the alpha-1 chain of type XIV collagen, a member of the fibril-associated collagen with interrupted triple helices (FACIT) family. COL14A1 is a non-fibrillar collagen expressed in connective tissues, particularly during development and tissue remodeling. It interacts with fibrillar collagens (e.g., types I and III) to regulate fibrillogenesis and stabilize extracellular matrix (ECM) architecture. COL14A1 contains a large N-terminal non-collagenous domain (NC3) and cysteine-rich regions critical for its structural and functional roles.

COL14A1 antibodies are essential tools for studying ECM dynamics, tissue fibrosis, and developmental biology. They are used in immunohistochemistry, Western blotting, and immunofluorescence to localize COL14A1 in tissues like skin, tendons, and blood vessels. These antibodies help investigate COL14A1's role in modulating cell-ECM interactions, mechanical properties of tissues, and pathological processes such as wound healing, cancer progression, and fibrotic diseases.

Research shows COL14A1 upregulation in stromal fibroblasts of certain tumors, correlating with poor prognosis. Antibodies specific to COL14A1 also aid in distinguishing its splice variants and post-translational modifications. Commercial antibodies are typically raised against recombinant protein fragments or synthetic peptides, with validation in human, mouse, and rat models due to high interspecies homology. Understanding COL14A1's biology through these antibodies contributes to therapeutic strategies targeting ECM remodeling.