Rabbit Polyclonal MYL1 Antibody

中文名： MYL1抗体
别名： Myosin light chain 1/3, skeletal muscle isoform, MLC1/MLC3, MLC1F/MLC3F, Myosin light chain alkali 1/2, Myosin light chain A1/A2, MYL1

货号: IPDX34512

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/500	Human,Mouse,Rat
ICC	1/25	Human,Mouse,Rat
FCM	1/25	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	Myosin light chain 1/3, skeletal muscle isoform, MLC1/MLC3, MLC1F/MLC3F, Myosin light chain alkali 1/2, Myosin light chain A1/A2, MYL1
Entrez GeneID	4632
WB Predicted band size	21.1kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This MYL1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-135 amino acids from the Central region of human MYL1.

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All lanes : Anti-MYL1 Antibody (Center) at 1:2000 dilution Lane 1: Human heart lysate Lane 2: Human skeletal muslce lysate Lane 3: Mouse skeletal muscle lysate Lane 4: Rat skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling MYL1 with P34512 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm and Weak Nucleus staining on Hela cell line. The nuclear counter stain is DAPI (blue).
Overlay histogram showing Hela cells stained with P34512(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34512, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
P34512 staining MYL1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

参考文献

以下是关于MYL1抗体的3篇参考文献的简要概括(注：内容基于领域内常见研究方向模拟整理，非真实文献)：

1. **文献名称**：*MYL1 as a Novel Biomarker in Rhabdomyosarcoma: Expression Analysis Using Immunohistochemistry*

**作者**：Smith A, et al.

**摘要**：研究通过MYL1抗体免疫组化分析，发现MYL1在横纹肌肉瘤中高表达，提示其可能作为该肿瘤的诊断标志物，并与患者预后相关。

2. **文献名称**：*Role of MYL1 in Skeletal Muscle Differentiation: Insights from Knockdown Models*

**作者**：Chen L, et al.

**摘要**：利用MYL1抗体检测基因沉默后肌细胞分化过程，证实MYL1通过调节肌球蛋白活性影响肌管形成，为肌肉再生研究提供依据。

3. **文献名称**：*MYL1 Overexpression in Prostate Cancer: Correlation with Aggressive Phenotypes*

**作者**：Park J, et al.

**摘要**：通过Western blot和免疫荧光技术结合MYL1抗体，揭示前列腺癌中MYL1过表达与肿瘤侵袭性及转移潜能正相关，提示其作为治疗靶点的潜力。

如需具体文献，建议通过PubMed或Google Scholar以“MYL1 antibody”、“MYL1 biomarker”等关键词检索最新研究。

背景信息

The MYL1 gene encodes myosin light chain 1 (MLC1), a regulatory component of myosin, a motor protein critical for muscle contraction and cellular motility. As part of the myosin light chain family, MYL1 is primarily expressed in skeletal muscle and plays a role in calcium-dependent regulation of myosin ATPase activity, influencing contractile force. Recent studies have highlighted its broader relevance beyond muscle physiology, particularly in cancer biology. MYL1 is aberrantly expressed in certain malignancies, including acute myeloid leukemia (AML) and solid tumors, where it may contribute to tumor cell proliferation, invasion, and metastasis. Antibodies targeting MYL1 are essential tools for investigating its expression patterns, subcellular localization, and functional roles in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research leveraging MYL1 antibodies has uncovered its potential as a diagnostic or prognostic biomarker in specific cancers, as well as its interaction with signaling pathways involved in cytoskeletal remodeling. Ongoing studies explore therapeutic opportunities by modulating MYL1 activity in diseases characterized by dysregulated cell motility, such as metastatic cancers.

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