Rabbit Polyclonal POLA1 Antibody

The POLA1 antibody targets the DNA polymerase alpha catalytic subunit, a crucial enzyme in initiating DNA replication. POLA1. encoded by the POLA1 gene, forms the core of the DNA polymerase α-primase complex, which synthesizes short RNA-DNA primers required for leading and lagging strand synthesis during replication. These primers enable DNA polymerases δ and ε to elongate strands, ensuring faithful genome duplication. POLA1 antibodies are widely used in research to study replication mechanisms, cell cycle regulation, and replication stress responses. They aid in detecting POLA1 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry, providing insights into its nuclear localization and cell cycle-dependent _expression (peaking in S-phase). Dysregulation of POLA1 is linked to genomic instability, developmental disorders, and cancers, making its antibody a tool for exploring replication-related pathologies. Recent studies also investigate POLA1's role in cancer therapy resistance, highlighting its potential as a biomarker or therapeutic target. Commercial POLA1 antibodies are typically developed in mouse or rabbit hosts, with validation in specific applications to ensure specificity for the ~180 kDa protein.