| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA |
| Entrez GeneID | 118 |
| WB Predicted band size | 81.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | This ADD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 428-462 amino acids from the Central region of human ADD1. |
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以下是3-4条关于 **ADD1抗体** 的参考文献及简要摘要：
1. **"Adducin: structure, function and regulation"**
- **作者**: Matsuoka Y., et al.
- **摘要**: 研究ADD1蛋白的结构及其在细胞骨架中的作用，利用特异性抗体验证其与spectrin的结合，阐明其在细胞膜形态维持中的功能。
2. **"Association of ADD1 gene polymorphism with hypertension"**
- **作者**: Cusi D., et al.
- **摘要**: 通过ADD1抗体进行Western blot分析，发现ADD1基因多态性可能影响钠离子转运，与原发性高血压的遗传易感性相关。
3. **"Adducin-1 is required for polarized vesicle trafficking and cell migration"**
- **作者**: Rafael Martínez-Marmol, et al.
- **摘要**: 利用ADD1抗体进行免疫荧光定位，揭示ADD1通过调控细胞膜蛋白运输参与癌细胞迁移和转移的分子机制。
4. **"Antibody-based detection of ADD1 in erythrocyte membranes"**
- **作者**: Bianchi G., et al.
- **摘要**: 开发高特异性ADD1单克隆抗体，验证其在人红细胞膜中的表达，并探讨其在膜稳定性及溶血性疾病中的潜在诊断价值。
(注：以上为模拟文献，实际引用需根据具体研究补充真实来源。)
The ADD1 (Adducin 1) antibody targets the alpha-adducin protein, a key component of the cytoskeleton. Adducins are a family of membrane-skeletal proteins (α, β, γ) that regulate actin filament assembly and stability by capping fast-growing ends and promoting spectrin-actin network formation. ADD1. encoded by the ADD1 gene, is predominantly expressed in erythrocytes and epithelial cells, where it maintains cell shape, membrane integrity, and cell-cell adhesion. Its function is modulated by phosphorylation (e.g., via protein kinase C) and calcium signaling, which influence its actin-binding activity.
Research on ADD1 gained momentum due to its association with hypertension and cardiovascular diseases. A notable polymorphism (Gly460Trp) in ADD1 has been linked to salt-sensitive hypertension in human studies, suggesting its role in renal sodium handling. Antibodies against ADD1 are essential tools for investigating these mechanisms, enabling detection of protein expression, localization, and post-translational modifications in tissues or cultured cells. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study cytoskeletal dynamics in conditions such as eryptosis (erythrocyte apoptosis), neurological disorders, and cancer metastasis. Commercially available ADD1 antibodies are typically raised in rabbits or mice, validated for specificity across species, and applied in both diagnostic and basic research contexts to unravel adducin's physiological and pathological roles.
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