Rabbit Polyclonal GDE1 Antibody

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     Anti-GDE1 Antibody (Center) at 1:1000 dilution + Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing Hela cells stained with P34499(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34499,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

**Background of GDE1 Antibody**

Glycerophosphodiester phosphodiesterase 1 (GDE1) is a transmembrane enzyme belonging to the glycerophosphodiester phosphodiesterase (GDPD) family, which hydrolyzes glycerophosphodiesters to produce glycerol phosphate and alcohols. GDE1 is implicated in cellular metabolism, lipid signaling, and membrane homeostasis. It is expressed in various tissues, including the brain, liver, and kidneys, and plays roles in neuronal differentiation, oligodendrocyte maturation, and metabolic regulation. Dysregulation of GDE1 has been linked to neurodegenerative diseases, cancer, and metabolic disorders.

GDE1 antibodies are immunological tools designed to detect and study the expression, localization, and function of the GDE1 protein. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate GDE1's role in physiological and pathological contexts. For example, studies have utilized GDE1 antibodies to explore its involvement in Alzheimer’s disease progression, hepatocellular carcinoma, and lipid metabolism anomalies.

As a transmembrane protein with enzymatic and signaling activities, GDE1's interaction partners and downstream pathways remain under active investigation. High-quality GDE1 antibodies are critical for validating its expression in disease models and understanding its mechanistic contributions, aiding the development of targeted therapies.