Rabbit Polyclonal CNOT4 Antibody

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     Anti-CNOT4 Antibody (N-Term) at 1:2000 dilution + Mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing U-2 OS cells stained with P34498(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34498,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P34498 staining CNOT4  in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Samples were incubated with primary antibody (1/250) for 1 hours at room temperature.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The CNOT4 antibody is a research tool designed to detect and study the CNOT4 protein, a subunit of the CCR4-NOT complex, which plays critical roles in gene expression regulation. The CCR4-NOT complex is evolutionarily conserved and involved in transcriptional and post-transcriptional processes, including mRNA deadenylation, decay, and translational repression. CNOT4. specifically, contains a RING domain that confers E3 ubiquitin ligase activity, enabling it to participate in ubiquitination pathways linked to protein degradation or modulation. It interacts with other subunits of the CCR4-NOT complex, such as CNOT1 and CNOT2/3. contributing to diverse cellular functions like stress response, cell cycle progression, and RNA metabolism.

Antibodies targeting CNOT4 are typically developed in rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies are validated for applications like Western blotting, immunoprecipitation, and immunofluorescence to assess CNOT4 expression, localization, and interactions. Studies using CNOT4 antibodies have elucidated its role in regulating transcription factors (e.g., p53), modulating ubiquitin-dependent signaling, and maintaining genomic stability. Dysregulation of CNOT4 has been implicated in diseases such as cancer and neurodegenerative disorders, highlighting its therapeutic relevance. Researchers rely on CNOT4-specific antibodies to explore its molecular mechanisms, validate knockout/knockdown models, and investigate its involvement in cellular homeostasis and disease pathways.