| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK |
| Entrez GeneID | 638 |
| WB Predicted band size | 18.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Bik BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-74 amino acids from human Bik BH3 Domain. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Bik BH3 Domain抗体的3篇示例参考文献(内容为虚构示例，仅用于演示格式)：
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1. **文献名称**: *"Characterization of a monoclonal antibody targeting the BH3 domain of pro-apoptotic Bik protein"*
**作者**: Suzuki H., et al.
**摘要**: 该研究开发了一种特异性识别Bik蛋白BH3结构域的单克隆抗体，验证了其在免疫印迹和免疫沉淀中的应用，并证明其可阻断Bik与抗凋亡蛋白Bcl-xL的相互作用，从而增强化疗诱导的肿瘤细胞凋亡。
2. **文献名称**: *"BH3 profiling in cancer cells using Bik-specific antibodies reveals apoptosis resistance mechanisms"*
**作者**: Certo M., et al.
**摘要**: 通过使用Bik BH3结构域抗体进行BH3分析，研究发现部分癌细胞中Bik表达下调与线粒体凋亡通路失调相关，提示Bik抗体可作为评估癌细胞凋亡敏感性的工具。
3. **文献名称**: *"Structural and functional analysis of Bik BH3 domain interactions via antibody-mediated epitope mapping"*
**作者**: Lindsten T., et al.
**摘要**: 利用Bik BH3抗体进行表位定位，揭示了该结构域与Bcl-2家族抗凋亡成员结合的分子机制，为设计靶向BH3结构域的小分子抑制剂提供了结构基础。
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(注：以上文献为模拟示例，实际引用请查询真实数据库如PubMed或Google Scholar。)
The Bik BH3 domain antibody is a tool used to study the pro-apoptotic protein Bik (Bcl-2-interacting killer), a member of the Bcl-2 protein family. Bik contains a conserved BH3 (Bcl-2 Homology 3) domain, which is critical for its function in promoting apoptosis by binding to anti-apoptotic Bcl-2 family members (e.g., Bcl-2. Bcl-xL) and neutralizing their survival signals. The BH3 domain facilitates these interactions through an amphipathic α-helical structure, enabling Bik to initiate mitochondrial outer membrane permeabilization, cytochrome c release, and caspase activation.
Antibodies targeting the BH3 domain of Bik are designed to specifically recognize this functional region, aiding in the detection, localization, and quantification of Bik in cellular or tissue samples. They are widely used in apoptosis research, cancer studies, and drug development, particularly in contexts where dysregulated apoptosis contributes to diseases like cancer or neurodegeneration. Such antibodies also help validate the role of Bik in BH3-only protein signaling and assess its interaction partners. Their development aligns with broader efforts to explore therapeutic strategies targeting BH3-mediated apoptosis, including the use of BH3 mimetics to overcome anti-apoptotic resistance in cancer. Specificity and validation (e.g., via knockout controls) are crucial to ensure accurate experimental outcomes.
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