| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nephrin, Renal glomerulus-specific cell adhesion receptor, NPHS1, NPHN |
| Entrez GeneID | 4868 |
| WB Predicted band size | 134.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Nephrin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1191-1219 amino acids from human Nephrin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Nephrin (Y1210)抗体的3篇参考文献的简要总结(注：文献为虚构示例，实际引用需核实)：
1. **"Tyrosine phosphorylation of Nephrin regulates podocyte cytoskeleton dynamics"**
*Author: Jones et al.*
摘要：研究揭示了Nephrin Y1210位点的酪氨酸磷酸化在足细胞骨架重组中的关键作用，通过特异性抗体证实其与Fyn激酶的相互作用，并调控细胞黏附信号通路。
2. **"Nephrin phosphorylation at Y1210 mediates diabetic nephropathy progression"**
*Author: Zhang et al.*
摘要：利用Y1210磷酸化抗体发现，糖尿病肾病模型中Nephrin Y1210磷酸化水平显著降低，导致足细胞损伤和蛋白尿，提示该位点可作为治疗靶点。
3. **"A novel antibody targeting Nephrin Y1210 reveals phosphorylation-dependent slit diaphragm organization"**
*Author: Tanaka et al.*
摘要：开发了针对Nephrin Y1210磷酸化表位的单克隆抗体，证明其磷酸化状态影响裂孔隔膜复合体的组装，并参与维持肾小球滤过屏障的完整性。
4. **"Nephrin tyrosine phosphorylation signaling in podocyte injury"**
*Author: Wang et al.*
摘要：通过Y1210特异性抗体检测发现，足细胞损伤模型中Nephrin磷酸化水平异常，激活下游MAPK通路，导致炎症反应和细胞凋亡。
建议通过PubMed或Web of Science以“Nephrin Y1210 phosphorylation antibody”为关键词检索真实文献。
Nephrin (Y1210) antibody is a phospho-specific antibody targeting tyrosine 1210 (Y1210) of the human nephrin protein, a critical component of the slit diaphragm in glomerular podocytes. Nephrin, encoded by the NPHS1 gene, plays a pivotal role in maintaining the structural and functional integrity of the kidney’s filtration barrier. Its phosphorylation at specific tyrosine residues, including Y1210. is essential for signaling pathways regulating podocyte morphology, cell-cell adhesion, and glomerular permeability. The Y1210 phosphorylation site is part of nephrin’s cytoplasmic domain, which interacts with adaptor proteins like Nck and activates downstream effectors such as PI3K/Akt and MAPK cascades. Dysregulation of nephrin phosphorylation has been implicated in proteinuric kidney diseases, including congenital nephrotic syndrome and diabetic nephropathy.
The Nephrin (Y1210) antibody is widely used in research to investigate phosphorylation-dependent nephrin signaling in podocyte injury models, kidney disease mechanisms, and therapeutic interventions. It is validated for applications like Western blotting, immunofluorescence, and immunohistochemistry, often in studies involving human or rodent tissues. By detecting phosphorylated Y1210. this antibody helps elucidate how nephrin-mediated signaling contributes to podocyte foot process dynamics and glomerular pathology. Its specificity makes it a valuable tool for exploring molecular pathways linking nephrin post-translational modifications to renal dysfunction and potential biomarkers or drug targets. Proper validation using knockout controls or phosphorylation inhibitors is recommended to ensure accurate interpretation.
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