| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4 |
| Entrez GeneID | 5166 |
| WB Predicted band size | 46.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified His-tagged PDK4 protein(Fragment) was used to produced this monoclonal antibody. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
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以下是3篇关于PDK4抗体的代表性文献(均为虚构示例，仅供格式参考)：
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1. **文献名称**: "PDK4 Antibody Validation in Cardiac Tissue: Implications for Ischemic Injury"
**作者**: Smith J, et al.
**摘要**: 本研究验证了一种新型PDK4单克隆抗体在心脏组织中的特异性，发现其在心肌缺血模型中PDK4蛋白表达显著上调，提示其可作为缺血性损伤的生物标志物。
2. **文献名称**: "PDK4 Expression in Colorectal Cancer: A Comparative Study Using IHC and Western Blot"
**作者**: Lee H, et al.
**摘要**: 通过免疫组化(使用PDK4抗体)和蛋白质印迹技术，发现PDK4在结直肠癌组织中高表达，且与患者生存率负相关，表明PDK4可能成为治疗靶点。
3. **文献名称**: "Development of a High-Specificity PDK4 Polyclonal Antibody for Metabolic Disease Research"
**作者**: Garcia R, et al.
**摘要**: 报道了一种高特异性兔源PDK4多克隆抗体的开发，验证其在肝脏和脂肪组织中的检测效能，并应用于肥胖模型中PDK4与胰岛素抵抗的机制研究。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“PDK4 antibody validation”或“PDK4 protein expression”获取最新研究。
PDK4 (pyruvate dehydrogenase kinase isoenzyme 4) is a mitochondrial enzyme that phosphorylates and inhibits the pyruvate dehydrogenase complex (PDC), a key regulator of glucose oxidation. By suppressing PDC activity, PDK4 shifts cellular metabolism from glucose utilization to fatty acid oxidation, a critical adaptation during fasting, exercise, or metabolic stress. Dysregulation of PDK4 has been implicated in various pathologies, including diabetes, cancer, and cardiovascular diseases. In cancer, PDK4 overexpression may promote the Warburg effect, sustaining tumor growth under hypoxic conditions. In metabolic disorders, elevated PDK4 levels are linked to insulin resistance and impaired glucose homeostasis.
PDK4 antibodies are essential tools for detecting and quantifying PDK4 protein expression in research. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study PDK4’s tissue-specific distribution, regulation, and role in disease mechanisms. High-quality PDK4 antibodies are validated for specificity, ensuring minimal cross-reactivity with other PDK isoforms (PDK1-3). Researchers employ these antibodies to investigate metabolic reprogramming in conditions such as obesity, cardiac hypertrophy, and cancer progression. Additionally, PDK4-targeted therapies, including small-molecule inhibitors, are under exploration, further highlighting the antibody’s utility in preclinical studies. Understanding PDK4’s function through antibody-based assays contributes to unraveling its therapeutic potential in metabolic and oncological disorders.
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