Rabbit Polyclonal ADAMTS4 Antibody

ADAMTS4 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 4) is a secreted extracellular matrix (ECM)-modifying enzyme involved in tissue remodeling, inflammation, and disease progression. It belongs to the ADAMTS family of zinc-dependent proteases, known for their roles in cleaving proteoglycans like aggrecan and versican. ADAMTS4 is particularly implicated in osteoarthritis, atherosclerosis, and cancer due to its ability to degrade cartilage components and modulate cell signaling pathways. Antibodies targeting ADAMTS4 are critical tools for studying its expression, localization, and functional contributions to pathophysiology. These antibodies are typically developed in model organisms (e.g., mice, rabbits) using immunogenic peptides or recombinant protein fragments corresponding to specific domains, such as the catalytic site or thrombospondin motifs. Monoclonal antibodies are preferred for consistent specificity in applications like Western blotting, immunohistochemistry, and ELISA, while polyclonal antibodies may offer broader epitope recognition. Researchers also employ neutralizing ADAMTS4 antibodies to inhibit enzymatic activity in vitro or in vivo, aiding mechanistic studies and therapeutic exploration. Recent interest in ADAMTS4 inhibitors for conditions like osteoarthritis has spurred the development of highly selective antibodies, often validated using knockout controls or activity assays. Proper validation ensures reliability in detecting both pro- and active forms of the enzyme, which is essential for understanding its regulation in health and disease.