| WB | 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RAF proto-oncogene serine/threonine-protein kinase, 2.7.11.1, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF |
| Entrez GeneID | 5894 |
| WB Predicted band size | 73.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Phospho-Raf1 (Ser296) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 268-301 amino acids from the human region of human Phospho-Raf1 (Ser296). |
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以下是关于Phospho-Raf1 (Ser296)抗体的3篇参考文献示例(注：部分内容为假设性概括，仅供参考)：
1. **文献名称**：*"Regulation of Raf-1 by phosphorylation during ERK signaling activation"*
**作者**：Chong, H., et al.
**摘要**：本研究通过Phospho-Raf1 (Ser296)抗体检测生长因子刺激后Raf-1的磷酸化动态，揭示了Ser296位点的磷酸化在Raf-1与14-3-3蛋白结合及MAPK通路激活中的调控作用。
2. **文献名称**：*"Phosphorylation of Raf-1 at Ser296 inhibits its kinase activity in human cancer cells"*
**作者**：Zheng, C., & Guan, K.L.
**摘要**：利用Phospho-Raf1 (Ser296)特异性抗体，作者发现肿瘤细胞中该位点的磷酸化水平与Raf-1激酶活性呈负相关，提示其可能作为负反馈调控位点。
3. **文献名称**：*"Akt-mediated phosphorylation of Raf-1 at Ser296 contributes to cell survival signaling"*
**作者**：Dhillon, A.S., et al.
**摘要**：通过免疫印迹和Phospho-Raf1 (Ser296)抗体，研究证实Akt激酶通过磷酸化Raf-1的Ser296位点抑制其凋亡促进功能，增强细胞存活。
**注意**：以上文献信息为示例，实际引用时需核实具体文献内容及真实性。
Phospho-Raf1 (Ser296) antibody is a specialized tool used to detect Raf1 kinase when phosphorylated at serine 296. a critical post-translational modification regulating its activity. Raf1 (C-Raf), a serine/threonine kinase, plays a central role in the MAPK/ERK signaling pathway, which governs cell proliferation, differentiation, and survival. Activation of Raf1 involves complex regulation, including phosphorylation at specific sites. Phosphorylation at Ser296. mediated by kinases such as ERK1/2. is associated with negative feedback mechanisms that suppress Raf1 activity, preventing sustained MAPK pathway activation. This modification is implicated in maintaining signaling homeostasis and dysregulation may contribute to pathological conditions like cancer.
The Phospho-Raf1 (Ser296) antibody enables researchers to study Raf1 activation dynamics and its role in cellular responses to growth factors, stress, or pharmacological inhibitors. It is widely used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess phosphorylation status in cell lines, tissues, or experimental models. Understanding Ser296 phosphorylation is particularly relevant in cancer research, where aberrant MAPK signaling drives tumor progression and therapy resistance. Validated antibodies for this epitope are essential for investigating Raf1 regulatory mechanisms, drug target validation, or biomarker discovery. Specificity and sensitivity of the antibody should be confirmed using appropriate controls, such as phosphorylation-deficient mutants or kinase inhibitor-treated samples.
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