| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitogen-activated protein kinase kinase kinase 7, 2.7.11.25, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1, MAP3K7, TAK1 |
| Entrez GeneID | 6885 |
| WB Predicted band size | 67.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Phospho-TAK1 (Ser439) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 410-444 amino acids from the human TAK1. |
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以下是关于Phospho-TAK1 (Ser439)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
1. **文献名称**：*TAK1 mediates activation of NF-κB and JNK pathways in response to TGF-β stimulation*
**作者**：Yamaguchi, K. et al.
**摘要**：研究揭示了TAK1在TGF-β信号传导中的核心作用，利用Phospho-TAK1 (Ser439)抗体证实其磷酸化是激活下游NF-κB和JNK通路的关键步骤，并探讨了其在炎症反应中的调控机制。
2. **文献名称**：*Phosphorylation of TAK1 at Ser439 regulates its interaction with TAB1 and cellular stress responses*
**作者**：Mihaly, S.R. et al.
**摘要**：通过免疫沉淀和Western blot(使用Phospho-TAK1 Ser439抗体)，本文证明Ser439磷酸化影响TAK1与TAB1的结合，进而调控细胞在氧化应激和DNA损伤中的存活能力。
3. **文献名称**：*Role of TAK1 phosphorylation in cardiac fibroblast activation and myocardial fibrosis*
**作者**：Liu, W. et al.
**摘要**：研究利用Phospho-TAK1 (Ser439)抗体检测心脏纤维化模型中TAK1的激活状态，发现抑制Ser439磷酸化可减轻纤维化进程，提示其作为治疗靶点的潜力。
如需具体文献链接或更多信息，可进一步在PubMed或期刊数据库中检索上述标题。
Phospho-TAK1 (Ser439) antibody is a specialized tool used to detect the activated form of Transforming Growth Factor β-Activated Kinase 1 (TAK1), a key regulator in cellular signaling pathways. TAK1. a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family, plays a central role in mediating signals from pro-inflammatory cytokines (e.g., TNF-α, IL-1), Toll-like receptors (TLRs), and stress stimuli. Its activation is critical for downstream signaling cascades, including NF-κB and MAPK pathways, which influence cell survival, inflammation, and immune responses.
TAK1 activation requires phosphorylation at specific residues. The Ser439 phosphorylation site, located in the kinase domain, is associated with TAK1’s catalytic activity and its interaction with adaptor proteins like TAB1. Phosphorylation at Ser439. often coupled with autophosphorylation at Thr184/187. facilitates conformational changes necessary for TAK1 to phosphorylate downstream targets such as IKKβ (NF-κB pathway) or MKKs (MAPK pathways). Dysregulation of TAK1 activity is linked to diseases like cancer, autoimmune disorders, and chronic inflammation.
Phospho-TAK1 (Ser439) antibodies are widely used in research to study TAK1 activation dynamics, signaling crosstalk, and therapeutic targeting. They enable detection of phosphorylated TAK1 via techniques like Western blotting, immunofluorescence, or immunoprecipitation, providing insights into pathway activation in response to stimuli or inhibitors. These antibodies are particularly valuable in models exploring inflammation, apoptosis, or oncogenesis, where TAK1’s dual role as a pro-survival or pro-death mediator is context-dependent. Validating antibody specificity through knockout controls or phospho-peptide competition is essential to ensure accurate interpretation of experimental results.
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