| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/250 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calmodulin, CaM, CALM1, CALM, CAM, CAM1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Calmodulin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-149 amino acids from the C-terminal region of human Calmodulin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇与Calmodulin抗体相关的经典文献摘要(基于公开信息整理)：
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1. **文献名称**: "Production and characterization of a monoclonal antibody to calmodulin"
**作者**: Van Eldik LJ, Burgess WH
**摘要**: 该研究报道了一种针对钙调蛋白(Calmodulin)的单克隆抗体的制备与特性分析。作者通过Western blot和免疫沉淀实验验证了该抗体的高特异性和灵敏度，并证明其适用于检测多种哺乳动物组织中的Calmodulin蛋白。
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2. **文献名称**: "Immunocytochemical localization of calmodulin in rat tissues using a monoclonal antibody"
**作者**: Harper JF, Cheung WY
**摘要**: 利用单克隆抗体技术，研究团队系统分析了钙调蛋白在大鼠不同组织中的定位分布。通过免疫组织化学(IHC)和免疫荧光技术，揭示了Calmodulin在神经、肌肉及内分泌系统中的差异表达模式。
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3. **文献名称**: "Cross-reactivity of anti-calmodulin antibodies with plant and fungal proteins"
**作者**: Roberts DM, Harmon AC
**摘要**: 本研究探讨了商业抗Calmodulin抗体在植物和真菌样本中的交叉反应性。结果发现部分抗体存在非特异性结合，提示在跨物种实验中需谨慎验证抗体适用性，以避免假阳性结果。
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这些文献涵盖了抗体开发、应用验证及潜在局限性，适用于实验设计参考。如需具体引用，建议通过PubMed或Google Scholar核对最新版本及DOI信息。
Calmodulin (CaM) is a highly conserved, calcium-binding protein found in all eukaryotic cells, playing a central role in calcium-mediated signaling pathways. By binding to Ca²⁺ via its EF-hand motifs, CaM undergoes conformational changes, enabling it to interact with and regulate a wide array of target proteins, including kinases, phosphatases, and ion channels. These interactions influence critical cellular processes such as muscle contraction, neurotransmitter release, cell cycle progression, and immune responses.
Antibodies targeting calmodulin are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are typically developed using immunogens derived from full-length CaM or specific peptide sequences, and they are available as monoclonal or polyclonal forms. Validation often involves techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP), with knockout controls to confirm specificity. Cross-reactivity with CaM homologs or structurally related proteins is a potential concern, requiring careful experimental design.
Calmodulin antibodies are widely used in neuroscience, cardiovascular research, and cancer studies. For example, they help investigate CaM's role in neurodegenerative diseases (e.g., Alzheimer’s) or its dysregulation in tumors. Additionally, these antibodies support drug discovery by screening compounds that modulate CaM-dependent pathways. Their versatility makes them indispensable for deciphering CaM's multifaceted roles in cellular signaling networks.
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